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Showing: 4261 - 4280 of 5043 results

  2. RANLS

    Plasmid
    #50843
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50843-plasmid-map-sequence-id-76292

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  3. GANLS

    Plasmid
    #50852
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

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  6. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  8. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177942-plasmid-map-sequence-id-348206

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  9. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  11. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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  16. Opto-M1R

    Plasmid
    #106069
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M1R; Opto-M1R; F11; 722)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-M1R (CHRM1 Bovine, Human)
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106069-plasmid-map-sequence-id-209711

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  17. NSP6-Flag

    Plasmid
    #210345
    Purpose
    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Article
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Insert
    SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210345-plasmid-map-sequence-id-418203

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  18. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    Use
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

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  19. GLuc-PM

    Plasmid
    #164783
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    Use
    Tags
    GLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164783-plasmid-map-sequence-id-326675

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Showing: 4261 - 4280 of 5043 results