Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 4261 - 4280 of 11285 results

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pcDNA3 Mito-Ras-LOCKR-S

Plasmid

#216501

Purpose

FRET-based reporter for Ras activity, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-deltaA1-Lock-VWF

Plasmid

#124824

Purpose

Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and Lock A2 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5-FRT-TO-Nsp13-HF_GC3opt

Plasmid

#157716

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 WT MYOC eGLuc2 FLAG

Plasmid

#192820

Purpose

Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

Depositor

John Hulleman

Article

Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

Insert

myocilin (MYOC Human)

Use

Tags

FLAG and enhanced Gaussia luciferase (M43I/M110I)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ost4-GFP-MTn

Plasmid

#154351

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

Plasmid

#112837

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

SH2 + SH3 (C-term) domains of Grb2

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH-8his (TB40/E)

Plasmid

#136445

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Use

Tags

Short GGSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular domain (a.a. 1-717). Codon-optimize…

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (T1462A)

Plasmid

#14130

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 T1462A (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T1462A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc card9 mutant

Plasmid

#16254

Depositor

William Kaelin

Article

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 mutant (CARD9 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S528A, T531A, T533A, S536A

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP DNAJB2a

Plasmid

#19497

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2a

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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