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Showing: 4241 - 4260 of 6029 results

  1. Desmoplakin I no force control (F40-based)

    Plasmid
    #118726
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1952
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    118726-plasmid-map-sequence-id-230443

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  2. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  3. LADL Bridge + Promoter Only Target

    Plasmid
    #127669
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Availability
    Academic Institutions and Nonprofits only
    127669-plasmid-map-sequence-id-249167

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  4. pVC-FUS-R521C

    Plasmid
    #175067
    Purpose
    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    changed Arginin 521 to Cystein; mVenus: C-Termina…
    Promoter
    CMV Promoter
    Availability
    Academic Institutions and Nonprofits only
    175067-plasmid-map-sequence-id-362347

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  12. Reb_206 aka PAS629

    Plasmid
    #71234
    Purpose
    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry
    Depositor
    Pamela Silver
    Article
    Polka et al ACS Synth Biol. 2016 Feb 3.
    Insert
    Reb locus
    Use
    Tags
    mNeonGreen to second copy RebB, soluble mCherry a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    71234-plasmid-map-sequence-id-244339

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  18. pHL 342

    Plasmid
    #53012
    Purpose
    PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    mCherry
    araC
    ompC
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -81 to +36 relative to start codon
    Promoter
    Plar, pLlacO-1, and pLtetO-1
    Availability
    Academic Institutions and Nonprofits only
Showing: 4241 - 4260 of 6029 results