Addgene

We narrowed to 8,506 results for: plat

Showing: 4221 - 4240 of 8506 results

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pRVV661

Plasmid

#108282

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_T

Plasmid

#74070

Purpose

pAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (T)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pAL-pck_T

Plasmid

#74068

Purpose

pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (T)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-puroR-p2a-HA-halo

Plasmid

#161979

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

puroR-p2a-HA-halo-GSlinker

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLSLGUS

Plasmid

#51517

Purpose

pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GUS
3kb filler sequence

Use

Plant t-dna plasmid

Tags

NLS and flag

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAMB_CAT-FspI

Plasmid

#61002

Purpose

T7-transcription template for CAT control mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

Chloramphenicol acetyltransferase

Use

Unspecified

Tags

Expression

Mutation

FspI site introduced after T7 terminator

Promoter

T7

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB8818

Plasmid

#126909

Purpose

Template for PCR amplification of HMG1from Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

YlHMG1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-UAS

Plasmid

#119001

Purpose

Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-let-7-A114-N40

Plasmid

#50563

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-let7-A114-N40

Use

Cloning vector

Tags

Expression

Mutation

Promoter

lac

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRVV584

Plasmid

#108280

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 delta gap

Plasmid

#49091

Purpose

Template for generating gene 60 mRNA, coding gap deletion

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and coding gap STOP have been deleted.

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRVV669

Plasmid

#108283

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRVV613

Plasmid

#108281

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_TTE1564

Plasmid

#61000

Purpose

T7-transcription template for Tte mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p35SZ

Plasmid

#51513

Purpose

pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant expression t-dna

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pYY_S1_400

Plasmid

#170470

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Kovari et al Methods Mol Biol. 2018;1665:317-340. doi

Insert

T7A1, Os, O1, lambda t1, in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS_1N_400

Plasmid

#170476

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

Insert

T7A1, O1, O-, Lambda t1 in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-blastR-p2a-v5-dL5

Plasmid

#161980

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

blastR-p2a-v5-dL5-GSlinker

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-v5-dL5-p2a-blastR

Plasmid

#161981

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GSlinker-V5-dL5-P2A-blastR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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