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pRSM32.2

Plasmid

#101635

Purpose

Encodes the fluorescent protein Rosmarinus under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Rosmarinus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRSM14

Plasmid

#101645

Purpose

Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

efasCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pME18S-F-R-hb2AR

Plasmid

#149367

Purpose

Expresses human b2AR in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

b2AR (ADRB2 Human)

Use

Tags

FLAG and rhodopsin

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

RPL4A

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

'long' CaMV35S

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
Pr-deCFP-MGapt

Plasmid

#67740

Purpose

OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deCFP-MGapt-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P04

Plasmid

#120015

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBabepuro-myc-RalB Q72L/A

Plasmid

#11121

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalB Q72L, RalA C-Terminus (RALA, RALB Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Q72L, RalB with C-Terminus removed and substitute…

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pIS1-miR-206 site

Plasmid

#25027

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-206 site

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
TB:2xP53_RE:MiniP

Plasmid

#133881

Purpose

2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

2 copies of the p53 DNA binding motif

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab35 WT

Plasmid

#37741

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab4 WT

Plasmid

#37690

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL ∆CC

Plasmid

#80268

Purpose

mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Use

Tags

Myc-GFP

Expression

Mammalian

Mutation

missing conserved coiled-coil (amino acids 721-74…

Promoter

CMV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-09

Plasmid

#119988

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Expression

Mutation

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

longCaMV35S

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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