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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-cMYC promoter

Plasmid

#114724

Purpose

reporter luciferase construct

Depositor

Martine Roussel

Article

Roussel et al Oncogene. 1994 Feb;9(2):405-15.

Insert

C-Myc promoter region

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-iRFP670-CaaX

Plasmid

#170464

Purpose

Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

iRFP670-CaaX

Use

Lentiviral

Tags

CaaX

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-EGFP

Plasmid

#122848

Purpose

N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Article

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Venus

Plasmid

#127346

Purpose

Expresses Venus constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Insert

Venus Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmAmetrine-DEVD-tdTomato

Plasmid

#18879

Purpose

Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Insert

mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)

Use

Tags

Expression

Mammalian

Mutation

mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pLEX_305-C-dTAG

Plasmid

#91798

Purpose

Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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v3em-Nterm-PE2max

Plasmid

#198734

Purpose

AAV genome encoding N-terminal PE2max

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Tags

Expression

Mutation

Promoter

Cbh

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169317

Purpose

Doxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pT7C-HSP90AB1

Plasmid

#177659

Purpose

Recombinant protein synthesis of human HSP90AB1

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSP90AB1 (HSP90AB1 Human)

Use

Tags

6xHis-Thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec23A

Plasmid

#166894

Purpose

mammalian expression of Sec23A tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

SEC23A (SEC23A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-Sarm1

Plasmid

#50707

Purpose

expression of Sarm1 in mammalian cells

Depositor

Yi-Ping Hsueh

Article

Chen et al J Cell Biol. 2011 May 16;193(4):769-84.

Insert

Sarm1 (Sarm1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only
TFORF3363

Plasmid

#144839

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR5A1 (NR5A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2890

Plasmid

#142168

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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