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Plasmid#197396PurposeExpresses SUMO-tagged mouse METAP1, residues 1-59DepositorInsertMETAP1 1-59 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free deadFPPS-NES
Plasmid#182492PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.DepositorInsertsFPPS
deadFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-L640AV646A_AD
Plasmid#148520PurposeMammalian Expression of HseIF4G1-L640AV646ADepositorInsertHseIF4G1-L640AV646A (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD
Plasmid#148521PurposeMammalian Expression of HseIF4G1-LIVVtoAAAADepositorInsertHseIF4G1-LIVVtoAAAA (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD
Plasmid#148522PurposeMammalian Expression of HseIF4G1-Y619AL624AL625ADepositorInsertHseIF4G1-Y619AL624AL625A (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chGrb2/eDHFR(69K6)-iRFP713
Plasmid#178854PurposeMammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713DepositorInsertGrb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
UseTagsExpressionMammalianMutationGrb2: Y160EPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s
Plasmid#172105PurposeMammalian expression of Gαs fused to miRFP670-eDHFR(69K6)DepositorInsertmiRFP670-eDHFR(69K6)-Gαs (GNAS Human)
UseTagsExpressionMammalianMutationGαs: C3SPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066
Plasmid#171135PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationA3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…PromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
px458-AMBRA1 #3
Plasmid#172607PurposeExpresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertgRNA against AMBRA1 (AMBRA1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7H126A
Plasmid#115180PurposeGateway cloning of PARK7H126A into any destination vectorDepositorInsertPARK7H126A (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.H126APromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7E163K
Plasmid#115181PurposeGateway cloning of PARK7E163K into any destination vectorDepositorInsertPARK7E163K (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.E163KPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2
Plasmid#115192PurposeLentiviral transduction and expression of PDHA2 into any mammalian cellDepositorInsertPDHA2WT (PDHA2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A
Plasmid#115196PurposeLentiviral transduction and expression of PDHA2S291A into any mammalian cellDepositorInsertPDHA2S291A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_PDHA2S291A/S293A
Plasmid#115198PurposeLentiviral transduction and expression of PDHA2S291A/S293A into any mammalian cellDepositorInsertPDHA2S291A/S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationp.S291A/S293APromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7V51G
Plasmid#115178PurposeGateway cloning of PARK7V51G into any destination vectorDepositorInsertPARK7V51G (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.V51GPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PARK7C53A
Plasmid#115179PurposeGateway cloning of PARK7C53G into any destination vectorDepositorInsertPARK7C53A (PARK7 Human)
UseGateway cloning into appropriate destination vect…TagsExpressionMutationp.C53APromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP-IQSEC1 v2N/v4C-WT
Plasmid#162022PurposeExpression of IQSEC1 chimeraDepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationv2N/v4CPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP-IQSEC1 v4N/v1C-WT
Plasmid#162024PurposeExpression of IQSEC1 chimeraDepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationv4N/v1CPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP-IQSEC1 v2N/v1C-WT
Plasmid#162023PurposeExpression of IQSEC1 chimeraDepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationv2N/v1CPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR
Plasmid#128507PurposeLentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only