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Plasmid#99614PurposeTo clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassetteDepositorInsertMb2Tomato
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LoxP1-Mb2YFP-2A (SO244)
Plasmid#99613PurposeTo clone gene of interest downstream of LoxP1-Mb2YFP-2A cassetteDepositorInsertMb2EYFP
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin GC
Plasmid#68818PurposeFlag tagged mammalian expression of GC domainDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationNILPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
1D3 HC
Plasmid#170670PurposeMammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)DepositorInsertHC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
1D3 LC
Plasmid#170669PurposeMammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)DepositorInsertLC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV_SEPT9_i1 D1-25-msfGFP
Plasmid#180333Purposemammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 D1-25PromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10TEV
Plasmid#159427Purposeempty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsTEV-protease-cleavable His10-tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
LoxP3-Mb2-HA-mTFP1-2A (SO157)
Plasmid#99615PurposeTo clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2ADepositorInsertMb2-HA-mTfp1
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LoxP2-H2B-EGFP-V5-2A (SO107)
Plasmid#99617PurposeTo clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2ADepositorInsertH2B-EGFP-V5
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-N (TRP1)
Plasmid#177797PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO R99A CoV-2 nsp1 3xFLAG
Plasmid#176061PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.DepositorInsertCoV-2 nsp1 (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LoxP3-HA-H2B-Cerulean-2A (SO104)
Plasmid#99618PurposeTo clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2ADepositorInsertHA-H2B-Cerulean
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pS6-C-hα2M-H6
Plasmid#128497PurposeExpresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.DepositorInsertA2M (F789-S1468) (A2M Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-C (TRP1)
Plasmid#177795PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh PH-mCh (VE5625)
Plasmid#139769PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.DepositorInsertmCherry fluorescent protein
UseTagsExpressionInsectMutationPromoterPH or p10Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2 pos ctrl
Plasmid#112029PurposeMode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)DepositorInsertNEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
UseTagsSplit mCherryExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin G
Plasmid#68817PurposeGeph G domain expression in mamalian cellsDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationNILPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR3-Flag-AuroraB KD (K106R)
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
UseTagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-DCRD-YFP
Plasmid#87450Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertDCRD (distal C-terminal regulatory domain) (CACNA1F Human)
UseTags6xHis-T7, FKBP, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSK_HUMAN_D0
Plasmid#79683PurposeThis plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCSK (CSK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only