Addgene

We narrowed to 6,564 results for: ski

Showing: 4181 - 4200 of 6564 results

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pAC4 Grb14-PIRSH2

Plasmid

#46438

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb14 (GRB14 Human)

Use

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 341-540

Promoter

Trc

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pPN013

Plasmid

#91587

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Utf1 gRNA

Plasmid

#128842

Purpose

gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Utf1 gRNA (Utf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK5

Plasmid

#53167

Purpose

Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp2

Plasmid

#157682

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPN107

Plasmid

#91636

Purpose

Express sgRNA targeting human KDM4A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KDM4A (KDM4A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp2-HF

Plasmid

#157684

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPN106

Plasmid

#91635

Purpose

Express sgRNA targeting human KDM4A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KDM4A (KDM4A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Grb7-PIR-SH2

Plasmid

#46443

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 334-532

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX HSH2-SH2

Plasmid

#46446

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

HSH2 (HSH2D Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-134

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-MDGA2

Plasmid

#82542

Purpose

Lentiviral vector for expressing HRP-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122025

Purpose

Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (CUG…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Promoter

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
TRCN0000072213

Plasmid

#78159

Purpose

shRFP control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

RFP

Use

RNAi

Tags

Expression

Mutation

Promoter

hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pPN060

Plasmid

#91593

Purpose

Express sgRNA targeting human CYP26B1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CYP26B1 (CYP26B1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN061

Plasmid

#91594

Purpose

Express sgRNA targeting human CYP26B1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CYP26B1 (CYP26B1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN064

Plasmid

#91597

Purpose

Express sgRNA targeting human DGKI

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DGKI (DGKI Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN065

Plasmid

#91598

Purpose

Express sgRNA targeting human DGKI

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DGKI (DGKI Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge