Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 4181 - 4200 of 11285 results

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pcDNA3.1(+)-HLA-cmyc-natT1R2

Plasmid

#113946

Purpose

mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 22-839

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P1-LoxP-Neo-N-miRFP670-C

Plasmid

#159434

Purpose

CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

Depositor

Jianhua Xing

Article

Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

Inserts

Neomycin
miRFP670

Use

CRISPR

Tags

3' linker pair 1, 5' linker pair 1, C t…

Expression

Mutation

Promoter

SV40

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (TB40/E)

Plasmid

#136449

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Use

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB6b

Plasmid

#19555

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB6b

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP1 N-terminal truncation

Plasmid

#22404

Purpose

Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1 (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pcDNA3

Plasmid

#51992

Purpose

mammalian expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-MAVS-M142A(KaganF61)

Plasmid

#51999

Purpose

Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

HA

Expression

Mammalian

Mutation

Met 142 to Ala

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-GFP-EBA175-Flag

Plasmid

#154970

Purpose

Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

insert codes for EBA175 residues 1284 to 1462

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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1479 pcDNA3 flag HA 14-3-3 gamma

Plasmid

#9000

Depositor

William Sellers

Insert

14-3-3 gamma (Ywhag Rat)

Use

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Myc-His RTN3A1

Plasmid

#31087

Depositor

Jimtong Horng

Article

Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

Insert

reticulon 3 (RTN3 Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB2a

Plasmid

#19550

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2a

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-LOC339287(MSL1)

Plasmid

#29762

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MSL1 (MSL1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Use

Tags

myc tag

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPH3

Plasmid

#19536

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 4-like (HSPA4L Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP1-S109A

Plasmid

#18981

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein S109A mutant (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Serine 109 changed to Alanine

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3 K50A

Plasmid

#78817

Purpose

To overexpress RIPK3 K50A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K50A

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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1432 pcDNA3 flag MAGI1c

Plasmid

#10714

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

MAGI-1 (Magi1 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
1481 pcDNA3 flag HA 14-3-3 zeta

Plasmid

#9002

Depositor

William Sellers

Insert

14-3-3 zeta (YWHAZ Bovine)

Use

Tags

HA and flag

Expression

Mammalian

Mutation

T226A

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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