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Plasmid#131251PurposeMammalian expression of N-terminally Myc-tagged USP7, minus the C-terminal 18 amino acidsDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_WT USP7 plasm…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikkb-FLAG(K44A)
Plasmid#23299DepositorInsertIkkbeta (IKBKB Human)
UseTagsFlagExpressionMammalianMutationK44APromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-WPRE
Plasmid#44358DepositorInsertRK-GFP (MAPK14 Human, Synthetic)
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 2557-2853)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-sfGFP
Plasmid#107057PurposeExpression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationK785RPromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_PP1-mCitrine
Plasmid#172472PurposeMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.DepositorInsertprolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
UseLentiviralTagsmCitrine and prolactin signal sequence (cleaved p…ExpressionMammalianMutationPromoterEF1alphaAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXJ42-p200 CUX1
Plasmid#100813PurposePlasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tagDepositorInsertp200 CUX1 (CUX1 Human)
UseNote that this plasmid does not have an sv40 oriTagsHA and MycExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330 Rosa26 Gu1 (LH416)
Plasmid#99629PurposePlasmid used to target the wildtype Rosa26 allele with Cas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikka-HA (K44M)
Plasmid#23297DepositorInsertIKK alpha (Chuk Mouse)
UseTagsHAExpressionMammalianMutationK44MPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-TEAD4
Plasmid#128335PurposeVector for LUMIER assayDepositorInsertTEAD4 (TEAD4 Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-Lambda2
Plasmid#104184PurposeLentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-sgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-Jun4A-Myc
Plasmid#47444Purposemammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)DepositorInsertJun 4A mutant (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationfour N-terminal residues phosphorylated by JNK, s…PromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEMS2159
Plasmid#70120PurposePromoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.DepositorInsertssAAV-MCS-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceMay 12, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCSCRET2(#323)
Plasmid#184058Purposecyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesisDepositorInsert6myc-CRE-ERT2
UseSynthetic BiologyTags6xMycExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4-S255D/T259E (GST)
Plasmid#21566DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
UseTagsGSTExpressionMammalianMutationSerine 255 to Aspartic Acid, Threonine 259 to Gl…PromoterAvailable sinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn (og codon)
Plasmid#174823PurposeMammalian expression of human MLH1dn (original codon usage)DepositorInserthuman MLH1dn (original codon usage) (MLH1 Human)
UseTagsExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT304 Ec07 Lenti pEF-attB LSR GFP
Plasmid#184947PurposeLentiviral delivery of recombinase landing padDepositorInsertEc07 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIE-hα2M-H6
Plasmid#128492PurposeExpresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.DepositorInsertA2M (A2M Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4-S255E/T259D (GST)
Plasmid#21565DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
UseTagsGSTExpressionMammalianMutationSerine 255 to Glutamic Acid, Threonine 259 to As…PromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mGreenLantern-KASH
Plasmid#191094PurposeTo express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmGreenLantern-KASH
UseAAV and Synthetic BiologyTagsmGreenLantern fused to the KASH domain of Nesprin…ExpressionMammalianMutationOptimizzation was done using the Human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEntr_Myc-BirA-G3-ER
Plasmid#173814PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseGateway shuttle vectorTagsMycExpressionMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only