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Plasmid#107592PurposeAn entry vector with cas9 -2A-mCherryDepositorInsertcas9-2A-mCherry
UseCRISPRTags2A-mCherryExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2
Plasmid#84603PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsODC1 destabilizing elementExpressionMutationPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Huc-Positron-ST
Plasmid#129265Purposepan neuronal expression of soma-localized Positron in zebrafishDepositorInsertPositron-ST
UseZebrafish expressionTagsExpressionMutationD92N, E199VPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-Tbait-UAS-Positron-ST
Plasmid#129266PurposeUAS-driven expression of Positron in zebrafishDepositorInsertPositron-ST
UseZebrafish expressionTagsExpressionMutationD92N, E199VPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAS:iGluSnfr.zf1
Plasmid#108356PurposeUAS driven variant of iGluSnFR for zebrafish expressionDepositorInsertUAS:[GlyRa1 secretion sequence]-[iGluSnFR]
UseZebrafish expressionTagsExpressionMutationPromoterlacAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2-hspDRv7_scGstlt
Plasmid#108870PurposescGESTALT V7 lineage barcode for zebrafishDepositorInsertscGESTALT lineage tracing target V7
UseTol2 integration into zebrafish genomeTagsExpressionMutationPromoterAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZE33 Prickle-1-GFPnopren-pCS2+
Plasmid#17074DepositorInsertPrickle-1 (prickle1a Zebrafish)
UseZebrafish expressionTagsGFPExpressionMutationPk1GFPnopren deletes the C-terminal six amino aci…PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#64237Purposeexpresses codon optimized Cas9 in zebrafishDepositorInsertcodon optimized Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3a
Plasmid#65612Purposeexpress soluble His-tagged zebrafish CSF3a/Gcsfa in E. coliDepositorInsertCsf3a (csf3a Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZE45 Dvl-2 pCS2P+
Plasmid#17086DepositorInsertDvl-2 (dvl2 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2-SauCas9
Plasmid#174040PurposeIn vitro transcription of SauCas9 mRNADepositorInsertSauCas9
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationR691A from wild-type Cas9PromoterT3Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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ZE41 Dapper-1 pCS2P+
Plasmid#17082DepositorInsertDapper-1 (dact1 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishTagsExpressionMutationPromotercx55.5Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1b
Plasmid#164957PurposeZebrafish kcnn1b gene CDSDepositorInsertkcnn1b (kcnn1b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
B3rec.zf1
Plasmid#108354PurposeZebrafish codon optimized B3 recombinase that acts on blown-out sitesDepositorInsertB3 recombinase
UseSynthetic BiologyTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZE42 myc-Dapper-1 pCS2P+
Plasmid#17083DepositorInsertDapper-1 (dact1 Zebrafish)
UseZebrafish expressionTagsmycExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:mCherry
Plasmid#68997Purposefor making transgenic fishDepositorInsertmhc2dab (mhc2dab Zebrafish)
UseZebrafish expressionTagsmcherryExpressionMutationPromotermhc2dabAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only