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Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218637PurposeProximity biotinylation control of OSBP(PH domain)DepositorInsertOSBP (OSBP Human)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPH domain onlyPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-msEEA1
Plasmid#214991PurposeGateway entry vector with mouse EEA1DepositorInsertEEA1 (Eea1 Mouse)
UseGatewayTags3xFLAGExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialMutationPromoterrrnB P1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218633PurposeProximity biotinylation of msNLRP3DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214996PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (TMEM115 Human)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214997PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (Tmem115 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-UbE2D1
Plasmid#15782DepositorInsertUbiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
UseTags6x His, T7 tag, and Xpress tag (enterokinase clea…ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Cdc34a
Plasmid#18674DepositorInsertCdc34A (CDC34 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N-Flag-HA-GFP
Plasmid#41034PurposeRetroviral expression of GFP in mammalian cellsDepositorInsertGFP
UseRetroviral; Gateway destination vectorTagsFlag, HA, and IRES PuroExpressionMammalianMutationPromoterLTR-driven expressionAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJT11F
Plasmid#165062PurposeIntroducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDA303
Plasmid#195199PurposeOverexpressing 6His-TetR in E. coli.DepositorInsertTetR
UseTags6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
msHook2 g1 lentiCRISPRv2-mCherry
Plasmid#218652PurposeKnockout vector for mouse Hook2DepositorInsertHook2 (Hook2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 msNLRP3 (STOP)
Plasmid#218638PurposeGateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)DepositorInsertNLRP3 (Nlrp3 Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
msHook2 g2 lentiCRISPRv2-mCherry
Plasmid#218653PurposeKnockout vector for mouse Hook2DepositorInsertHook2 (Hook2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT9RFR
Plasmid#165067PurposeIntroducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiaeDepositorInsertT-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE-FLAG-APEX2-GFP
Plasmid#175757PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only