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Plasmid#132695PurposepRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-vhhGFP4-hIgG1-Fc
Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-myc-BirA
Plasmid#109424PurposeBacterial expression of biotin ligase (BirA) with an N-terminal myc epitopeDepositorInsertBirA (birA )
UseTagsmycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-convex
Plasmid#132696PurposepRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curveDepositorInsertSb_convex including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Gal4DBD-GBP2
Plasmid#49439PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP2-Gal4 DNA binding domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_ALFA-tag-SNAP
Plasmid#136628PurposePlasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)
Plasmid#136627PurposePlasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-LAMA-R59
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_Flag-myc-NES-Tir1
Plasmid#117717PurposemRNA synthesis of Flag-myc-NES-TIR1 for injectionDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
UseTagsFlag-Myc-NESExpressionMammalianMutationcodon optimized TIR1 for murine expressionPromoterSP6Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN-dGBP1-TagBFP
Plasmid#80087PurposeMMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection markerDepositorInsertdGBP1-TagBFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p65AD-GBP6
Plasmid#49440PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP6-p65 transactivation domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rTetRDBD-GBP1
Plasmid#50791PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertrTetR DNA binding domain-GBP1 fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP6-10gly-VPminx4
Plasmid#50796PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP6-VPminx4 activation domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES EGFP
Plasmid#136618PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-F98-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP7-p65AD
Plasmid#49441PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP7-p65 transactivation domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-BC2T
Plasmid#108970Purposeexpresses tubulin-BC2-tag in mammalian cellsDepositorInserta-tubulin
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GBP1-10gly-lexADBD
Plasmid#50795PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP1-lexA DNA binding domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES EGFP
Plasmid#136620PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVIM-BC2T
Plasmid#108967Purposeexpresses vimentin-BC2-tag in mammalian cellsDepositorInsertVimentin
UseTagsBC2TExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only