Addgene

We narrowed to 860 results for: multisite gateway

Showing: 401 - 420 of 860 results

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IO102: pMVP (R4-R3) eGFP open

Plasmid

#121731

Purpose

pMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HJ601: pMVP (R4-R3) mCherry open

Plasmid

#121733

Purpose

pMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO601: pMVP (R4-R3) eCFP open

Plasmid

#121735

Purpose

pMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO401: pMVP (R4-R3) eYFP open

Plasmid

#121737

Purpose

pMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KH305: pMVP (R4-R3) βgal open

Plasmid

#121739

Purpose

pMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

βgal (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF801: pMVP (L1-R5) cTnT promoter

Plasmid

#121696

Purpose

pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

cTnT promoter (Cardiomyocyte specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU801: pMVP (L5-L4) mCherry

Plasmid

#121709

Purpose

pMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU901: pMVP (L5-L4) eCFP

Plasmid

#121711

Purpose

pMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KV001: pMVP (L5-L4) eYFP

Plasmid

#121713

Purpose

pMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HB501: pMVP (L1-L4) RIP

Plasmid

#121688

Purpose

pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Rat Ins1 promoter (RIP; β-cell specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi

Plasmid

#27320

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

zebrafish ubiquitin promoter (ubb Zebrafish)

Use

Multisite gateway 5' entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-TagRFP-t35s

Plasmid

#118386

Purpose

Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p-TagRFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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p2R3z-BASI-ccdB-BSAI

Plasmid

#118389

Purpose

Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

ccdB

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_CBF-tdTomato_L3-L2

Plasmid

#113708

Purpose

Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

CBF (notch dependent promoter)

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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p2R3z-AtU3b-tRNA-ccdB-gRNA

Plasmid

#118390

Purpose

Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtU3b promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWR

Plasmid

#112900

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge