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Plasmid#172608PurposeExpresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.DepositorInsertgRNA against AMBRA1 (AMBRA1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-E381A
Plasmid#35058DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; E381A mutationPromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-E272A
Plasmid#35057DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; E272A mutationPromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-E222A
Plasmid#35056DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; E222A mutationPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-T220A
Plasmid#35055DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; T220A mutationPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-Y218A
Plasmid#35054DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionMutationContains HinA domain: aa 192-393; Y218A mutationPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4-delta-kinase-PY1 mutant
Plasmid#17801DepositorInsertErbB4 (ERBB4 Human)
UseTagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment, amino acids 988-1292, …PromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
p53 (dominant negative R175H mutant)-pcw107-V5
Plasmid#64638Purpose3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insertDepositorInsertTP53 (transcript variant 3) (TP53 Human)
UseLentiviralTagsV5ExpressionMutationR175HPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-codon optimized catalytically inactive PHGDH
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneUseTagsFokI nuclease (ELD)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneUseTagsFokI nuclease (KKR)ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ratiometric FPX sensor for ERK kinase activity
Plasmid#60974PurposeFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.DepositorInsertddRFP A-WW domain-ERK substrate-ddFP B
UseTagsERK substrate is before ddFP copy B, WW domain is…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)
Plasmid#139844PurposeLentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)DepositorInsertAMPK-Kinase-dead (Prkaa2 Rat)
UseLentiviralTagsGSTExpressionMutationK47RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA (pcDNA3-FLAG)
Plasmid#35062DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsFLAG and HisExpressionMammalianMutationContains HinA domain: aa 192-393PromoterCMVAvailable sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
Mouse Ism1 with C-terminal Myc-6X-his tag
Plasmid#173046PurposeExpresses Ism1 in mammalian cellsDepositorInsertIsm1 (Ism1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FabF (KASII) pet28 N-terminal His Tag
Plasmid#75017PurposeExpresses the ketosynthase FabF/KASII from the E. coli fatty acid synthaseDepositorInsertFabF (fabF E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K
Plasmid#187273PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758KDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationAnillin A740D, E758KPromoterU6, CMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 C-terminal half
Plasmid#74603Purposeexpresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationamino acids 389-523 of LIC1PromoterSFFVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q GFP
Plasmid#32127DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationE171QPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only