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Plasmid#210565PurposeExpresses Spycatcher003 on the cell surface using human CD52 hingeDepositorInsertSpycatcher003 fused to human CD52 hinge
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTGV-Express-CAT SMR5916
Plasmid#28039DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-T172A-P-R
Plasmid#189854PurposeCre-inducible activation of cat-T172A for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-catT172A
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mCherry-FLAG:cat
Plasmid#211831PurposePlasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.DepositorInsertmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-NanoLuc-BRAF-CAT
Plasmid#194772PurposeMammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.DepositorInsertBRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMV51Available sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP7 (catalytic domain)
Plasmid#16360DepositorInserthuman SENP7 catalytic domain (SENP7 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 640 to 984 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mTagBFP2-SOScat
Plasmid#178866PurposeMammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2DepositorInsertFKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXJ40-GFP-iBox-PAK4cat
Plasmid#190179Purposeexpresses GFP-tagged iBox-PAK4catDepositorInsertGFP-iBox-PAK4cat
UseTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 beta-catenin Y654F
Plasmid#16072DepositorInsertbeta-catenin (CTNNB1 Chicken)
UseTagsEGFPExpressionMammalianMutationY654F, *(see below)PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD∆cat
Plasmid#81054Purposebacterial expression of catalytic dead catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057 with histid…PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE49 pt Beta-catenin-myc
Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationfour point mutations introduced into the GSK-3 ph…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 beta-catenin Y654E
Plasmid#16073DepositorInsertbeta-catenin (CTNNB1 Chicken)
UseTagsEGFPExpressionMammalianMutationY654E, *(see below)PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXJ40-Flag-iBox-PAK4cat
Plasmid#190178Purposeexpresses Flag-tagged iBox-PAK4catDepositorInsertiBox-PAK4cat
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Beta Catenin (Xenopus)
Plasmid#13434DepositorInsertBeta Catenin (ctnnb1.L Frog)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mCherry-Vav2cat
Plasmid#178865PurposeMammalian expression of Vav2(DH-PH-CRD) fused to FKBP-mCherryDepositorInsertFKBP-mCherry-Vav2(DH-PH-CRD) (Vav2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2MT-mbeta-catenin delta6
Plasmid#100883PurposeMammalian expression of mouse beta-catenin truncationDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseTags6xMycExpressionMammalianMutationtruncated Ctnnb1 489-781aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2MT-mbeta-catenin delta7
Plasmid#100884PurposeMammalian expression of mouse beta-catenin truncationDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseTags6xMycExpressionMammalianMutationtruncated Ctnnb1 668-781aaPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only