Skip to main content
Addgene
Showing: 401 - 420 of 16979 results

  1. pET27b+R

    Plasmid
    #188245
    Purpose
    Empty backbone vector for protein expression in Escherichia coli and his-tag purification
    Depositor
    Yiping Qi
    Article
    Fang et al Methods Mol Biol. 2023;2653:153-171. doi
    Type
    Empty backbone
    Use
    Tags
    6xHis tag and TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188245-plasmid-map-sequence-id-368371

    Enlarge

  2. pJK599

    Plasmid
    #72524
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72524-plasmid-map-sequence-id-132609

    Enlarge

  3. pJK618

    Plasmid
    #72535
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72535-plasmid-map-sequence-id-132626

    Enlarge

  9. pJK571

    Plasmid
    #75379
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glycine-209 to aspartate and synthetic ge…
    Promoter
    n/a
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    75379-plasmid-map-sequence-id-142756

    Enlarge

  10. pJK040 (pET-PurQ-A128T)

    Plasmid
    #73840
    Purpose
    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Article
    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A128T mutant
    Promoter
    T7
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73840-plasmid-map-sequence-id-136936

    Enlarge

  12. pJK598

    Plasmid
    #72523
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72523-plasmid-map-sequence-id-132608

    Enlarge

  13. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

    Enlarge

  14. pJK613

    Plasmid
    #72534
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72534-plasmid-map-sequence-id-132623

    Enlarge

  15. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

    Enlarge

  18. pJB158

    Plasmid
    #202601
    Purpose
    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.
    Depositor
    Athanasios Typas
    Article
    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202601-plasmid-map-sequence-id-412308

    Enlarge

  19. pGAN147

    Plasmid
    #129202
    Purpose
    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone
    Depositor
    Ahmad Khalil
    Article
    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129202-plasmid-map-sequence-id-255858

    Enlarge

  20. TR-Csy4-H29A

    Plasmid
    #80602
    Purpose
    Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.
    Depositor
    Aravind Asokan
    Article
    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12
    Insert
    Csy4-H29A
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    His29 mutated to Ala
    Promoter
    CBA
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    80602-plasmid-map-sequence-id-152080

    Enlarge

Showing: 401 - 420 of 16979 results