Addgene

We narrowed to 433 results for: TagRFP

Showing: 401 - 420 of 433 results

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pTSTEP

Plasmid

#72334

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-EGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTSTEP-SNAPf

Plasmid

#72335

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-SNAPf

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AA267

Plasmid

#216065

Purpose

Fragmid fragment: (2A Selection) TagRFP657

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

P2A_v1.1; TagRFP657_v1.1; Stop

Use

Fragment

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

Plasmid

#212961

Purpose

Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_backbone

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

Plasmid

#212962

Purpose

Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_non-targeting spacer

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

Plasmid

#212963

Purpose

Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_GFP targeting spacer

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pEx_Hsp70::attBmut

Plasmid

#48878

Purpose

PhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pGlnA-Ypet

Plasmid

#98278

Purpose

mammalian expression of GlnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

GlnA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pFtnA-Ypet

Plasmid

#98280

Purpose

mammalian expression of FtnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pFtnA-Halo

Plasmid

#98281

Purpose

mammalian expression of FtnA-Halo

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

Halo-tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAP-FtnA

Plasmid

#98282

Purpose

mammalian expression of SNAP-FtnA

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

SNAPf-tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pDpsTE-Ypet

Plasmid

#98277

Purpose

mammalian expression of DpsTE-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Dps

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pYpet-FsaA

Plasmid

#98279

Purpose

mammalian expression of Ypet-FsaA

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FsaA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-GR-ICUE2

Plasmid

#173017

Purpose

Improved green-red FRET based cAMP indicator.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-ICUE2

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSNAPf-Nup43

Plasmid

#98276

Purpose

mammalian expression of SNAPf-Nup43

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup43 (NUP43 Human)

Use

Tags

SNAPf-tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pNup133-Ypet

Plasmid

#98283

Purpose

mammalian expression of Nup133-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup133 (NUP133 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyn-GR-AKAR3

Plasmid

#173016

Purpose

Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Lyn-GR-AKAR3

Use

Tags

EGFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
AA279

Plasmid

#216001

Purpose

Fragmid fragment: (Selection) fluorescent reporter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Start; TagRFP_v1.1

Use

Fragment

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge