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Plasmid#160515PurposeExpression of inserted ILY variantDepositorInsertILY-WT-Y336W
UseTagsHis-TagExpressionBacterialMutationY336WPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
Plasmid#50032PurposeGalactose-inducible expression of ER-luminal S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
UseTagsHA-HDELExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0016
Plasmid#117526PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0DepositorInsertSp-eCas9 1.0 (with stop codon)
UseCRISPRTagsExpressionMutationK810A, K1003A, R1060APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P4_AtU626Ter26
Plasmid#191772PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P7_AtU626Ter26
Plasmid#191775PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P1_AtU626Ter26
Plasmid#191769PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P2_AtU626Ter26
Plasmid#191770PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P5_AtU626Ter26
Plasmid#191773PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P6_AtU626Ter26
Plasmid#191774PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID2S
Plasmid#188647PurposeExpressing Target-AID2S in yeastsDepositorInsertnCas9-AID2S-UGI
UseTagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-N342W
Plasmid#160536PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-N342W
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, N342WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-L340W
Plasmid#160535PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-L340W
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, L340WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-N338W
Plasmid#160534PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-N338W
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, N338WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-Y336W
Plasmid#160533PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-Y336W
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, Y336WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-charge+
Plasmid#160531PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-charge+
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, Y336K, L340KPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock
Plasmid#160530PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock
UseTagsHis-TagExpressionBacterialMutationY208C, D322CPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-N342W
Plasmid#160529PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-N342W
UseTagsHis-TagExpressionBacterialMutationA221C, N404C, N342WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-L340W
Plasmid#160528PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-L340W
UseTagsHis-TagExpressionBacterialMutationA221C, N404C, L340WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-N338W
Plasmid#160527PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-N338W
UseTagsHis-TagExpressionBacterialMutationA221C, N404C, N338WPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only