Showing: 401 - 420 of 1382 results
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Plasmid#140568PurposeExpresses xCas9 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationA262T R324L S409I E480K E543D M694I E1219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET21b - S. thermophilus DltB
Plasmid#132607Purposeexpresses S. thermophilus DltB in E. coliDepositorInsertDltb
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP
Plasmid#86987PurposeFor cloning and expression of sgRNA together with expression of Cas9 and TagRFPDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationR691A from wild-type Cas9PromoterT3AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3'UTR-ST2-com-vector
Plasmid#136269PurposeExpresses SpCas9 and sgRNA ScaffoldDepositorInsertSpCas9 and sgRNA scaffold
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSEVA421-Cas9tr
Plasmid#138709PurposePlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenesDepositorInserttracrRNA-Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRC319
Plasmid#61272PurposeAlso referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)
Plasmid#50031PurposeExpression of S. pyogenes SrtA at the plasma membrane in mammalian cellsDepositorInsertPM-SrtA(strep)
UseTagsC-HAExpressionMammalianMutationPromoterpCMVAvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
Plasmid#50032PurposeGalactose-inducible expression of ER-luminal S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
UseTagsHA-HDELExpressionYeastMutationPromoterpGALAvailabilityAcademic Institutions and Nonprofits only -
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCas9-polyA
Plasmid#72602Purposeexpress hCas9 byT7 promoter with polyadenine tailsDepositorInsertCas9-polyadenine
UseTags81-bp polyadenylationExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMJ920
Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRPaCas9
Plasmid#111819PurposeExpresses Cas9 in T. brucei (2T1) cellsDepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL90005
Plasmid#117520PurposeLevel0 Golden Gate part: CDS, SpCas9-h (no stop codon)DepositorInsertSpCas9-h (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0009
Plasmid#117522PurposeLevel0 Golden Gate part: CDS, SpCas9-p (no stop codon)DepositorInsertSpCas9-p (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0001
Plasmid#117523PurposeLevel0 Golden Gate part: CDS, SpCas9-DE (no stop codon)DepositorInsertSpCas9-DE (no stop codon)
UseCRISPRTagsExpressionMutationD1135EPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0014
Plasmid#117524PurposeLevel0 Golden Gate part: CDS, SpCas9-KADepositorInsertSpCas9-KA (with stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0015
Plasmid#117525PurposeLevel0 Golden Gate part: CDS, SpCas9-KA (no stop codon)DepositorInsertSpCas9-KA (no stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 401 - 420 of 1382 results