-
Plasmid#156409PurposepCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12DDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseTagsmTagBFP2ExpressionMammalianMutationmutant KRAS4A-G12DPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156410PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12VDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationmutant KRAS4A-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
Plasmid#134612PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 375 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA
Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)
Plasmid#156407PurposepCS2 CMV expression vector driving EGFP fused human KRAS4B-G12VDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseTagsEGFPExpressionMammalianMutationmutant KRAS4B-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M85-303: CMV51p> Halotag-Hs.KRAS4a
Plasmid#159680PurposeMammalian protein expression of KRAS4a-Halotag fusionDepositorInsertHalotag-Hs.KRAS4a (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1
Plasmid#176537PurposeNon-protein coding locus components from KraIscB-1DepositorInsertnon-coding locus components from KraIscB-1 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196080Purpose(Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Blasticidin S deaminase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027
Plasmid#134611PurposeMammalian expression of mouse KIF5A 378-1027DepositorInsertKIF5A (Kif5a Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196074Purpose(Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M48-303: CMV51p> Halotag-Kras4aHVR
Plasmid#159684PurposeMammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-Kras4aHVR (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R703-M80-303: CMV51p> Halotag-Kras4bHVR
Plasmid#159683PurposeMammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-Kras4bHVR (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: Venus-oLID-CAAX (from KRas4B)
Plasmid#60412PurposeMammalian Expression of Venus-oLID-CAAXDepositorInsertVenus-oLID-CAAX
UseCre/Lox and LentiviralTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156406PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseGateway cloningTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)
Plasmid#156414Purposekdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseZebrafish transgenesisTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-hUbC-dCas9-KRAB-T2a-Puro
Plasmid#225999PurposeTo establish stable dCas9-KRAB knock-in lineDepositorInserthumanized dCas9-KRAB T2A Puro
UseSleeping beauty transposonTagsExpressionMammalianMutationD10A and H840APromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
Plasmid#225488PurposeTet-on lentiviral vector for controlled expression of GDNFDepositorInsertTetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
UseLentiviralTagsExpressionMammalianMutationPromoterMouse phosphoglycerate kinase 1Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2
Plasmid#223149PurposeExpression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G121C/G13A-nick sgRNA
Plasmid#214099PurposeLentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3DepositorInsertKRAS G12C or G13A nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only