Addgene

We narrowed to 425 results for: DHFR

Showing: 401 - 420 of 425 results

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PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

PfU6

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-F98 (for GFP)

Plasmid

#130714

Purpose

Plasmid encoding the bacterial expression of LAMA-F98 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-F98

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98

Plasmid

#130704

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-G97 (for GFP)

Plasmid

#130715

Purpose

Plasmid encoding the bacterial expression of LAMA-G97 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-G97

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97

Plasmid

#130705

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_LAMA-N95 (for GFP)

Plasmid

#130716

Purpose

Plasmid encoding the bacterial expression of LAMA-N95 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-N95

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX_Snifit

Plasmid

#119043

Purpose

Plasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX_Snifit

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44L H45Q

Promoter

pBAD

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-F98 IRES EGFP

Plasmid

#130710

Purpose

Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)

Plasmid

#117908

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-G97 IRES mYFP

Plasmid

#130713

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS IRES mYFP

Use

Tags

NLS and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-F98 IRES mYFP

Plasmid

#130712

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS IRES mYFP

Use

Tags

NLS and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97 IRES EGFP

Plasmid

#130707

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES EGFP

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-S98 (for p24)

Plasmid

#130717

Purpose

Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-S98

Use

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX-u36_Snifit

Plasmid

#119045

Purpose

Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX-u36_Snifit

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

36TAG R44L H45Q

Promoter

pBAD

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX-u51_Snifit

Plasmid

#119046

Purpose

Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX-u51_Snifit

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

51TAG R44L H45Q

Promoter

pBAD

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-MTX-u23_Snifit

Plasmid

#119044

Purpose

Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX-u23_Snifit

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

23TAG R44L H45Q

Promoter

pBAD

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

Plasmid

#130708

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

Plasmid

#130709

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 420 of 425 results

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