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Plasmid#126905PurposeTemplate for PCR amplification of XdCrtYBDepositorInsertXdCrtYB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1F5
Plasmid#165064PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminatorDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYUB28b-MCS-optimized
Plasmid#89179Purposemycobacteria shuttle vector - pYUB28b with a single change to the MCSDepositorTypeEmpty backboneUseMycobacteria shuttle vectorTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB8740
Plasmid#126906PurposeTemplate for PCR amplification of XdCrtIDepositorInsertXdCrtI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8741
Plasmid#126907PurposeTemplate for PCR amplification of XdCrtEDepositorInsertXdCrtE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII TAG392
Plasmid#105848Purposeexpresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 392PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbE7c-XAacxnT (JBEI-14118)
Plasmid#100961PurposeXAacx operon in pBbE7cDepositorInsertXAacx operon
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pProm11_BCD1-GFP
Plasmid#80656PurposePromoter11 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII TAG415
Plasmid#105847Purposeexpresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 415PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII TAG348
Plasmid#105844Purposeexpresses E. coli HPII catalase with TAG stop codon at site 348 and N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 348PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-HPII TAG283
Plasmid#105843Purposeexpresses E. coli HPII catalase with TAG stop codon at site 283 and N-terminal 6x his tagDepositorArticleInserthydroperoxidase II catalase
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 283PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL
Plasmid#139798Purposeexpresses biosynthetic enzyme 4CLDepositorInsert4CL
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pProm9_BCD1-GFP
Plasmid#80655PurposePromoter9 with BCD1-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLU
Plasmid#64174Purposeharbours marker genes LEU2 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
BB1_P_12_asn_coxA
Plasmid#90290PurposeBB1 promoter coxA gene of Aspergillus niger between FS1 and FS2DepositorInsertcoxA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_L_23_syn_BsaI
Plasmid#89915PurposeLinker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHK
Plasmid#64187Purposeharbours marker genes HIS3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD20-GFP
Plasmid#80651PurposePromoter1 with BCD20-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pProm1_BCD21-GFP
Plasmid#80652PurposePromoter1 with BCD21-gfpDepositorInsertGFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationNONEPromoterconstitutive promoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLUK
Plasmid#64184Purposeharbours marker genes LEU2, URA3 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU
Plasmid#64172Purposeharbours marker genes HIS3 and URA3DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLM
Plasmid#64175Purposeharbours marker genes LEU2 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXD80RFP-pXD68Kan2-RFP
Plasmid#225329PurposeRSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistanceDepositorInsertRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-Fab-HC-Halo
Plasmid#230061PurposeExpresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.DepositorInsertAnti-GluA2 Fab heavy chain fragment
UseTagsHaloTagExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKJ247
Plasmid#228714PurposeMammalian expression of U6 epPsaCas12f RNF2 targeting guideDepositorArticleInsertu6-RNF2 targeting guide
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PT7extended-MGapt-UTR1-deGFP
Plasmid#227646PurposepT7-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis with extended T7 promoter.DepositorInsertMG aptamer (MGapt) and deGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12717
Plasmid#212691Purposeintegration vector for three gene cassette with integration site C3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12708
Plasmid#212678PurposeGPD promoterDepositorInsertGPD
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13090
Plasmid#212679PurposeLip2 terminatorDepositorInsertLip2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12718
Plasmid#212692Purposeintegration vector for three gene cassette with integration site F3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12715
Plasmid#212689Purposeintegration vector for three gene cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13017
Plasmid#212693Purposeintegration vector for three gene cassette with integration site of the gene 4HPPD locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12716
Plasmid#212690Purposeintegration vector for three gene cassette with integration site E4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12714
Plasmid#212688Purposeintegration vector for three gene cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_Q33STS
Plasmid#191292PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R166QTS
Plasmid#191311PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_WTTS
Plasmid#191310PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_Q33STS
Plasmid#191313PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only