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Showing: 601 - 630 of 3290 results

  1. pLenti-CMV-Myr-Pink Flamindo

    Plasmid
    #204669
    Purpose
    Mammalian expression of membrane-bound Pink Flamindo for cAMP imaging
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Insert
    Myr-Pink Flamindo
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    204669-plasmid-map-sequence-id-407794

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  2. pLenti-CMV-paGFP-bPAC

    Plasmid
    #204670
    Purpose
    Mammalian expression of bPAC tagged with paGFP for cAMP production
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Insert
    paGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    204670-plasmid-map-sequence-id-407792

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  4. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    FKBP-ICUE3-NLS
    Use
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  6. Epac-S-H90

    Plasmid
    #170340
    Purpose
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170340-plasmid-map-sequence-id-335919

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  7. KG#203

    Plasmid
    #110880
    Purpose
    Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110880-plasmid-map-sequence-id-212521

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  8. Epac-S-H81

    Plasmid
    #170339
    Purpose
    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170339-plasmid-map-sequence-id-344640

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  9. Epac-S-H332

    Plasmid
    #228250
    Purpose
    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228250-plasmid-map-sequence-id-451844

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  11. pLenti-CMV-paGFP-bPAC-CAAX

    Plasmid
    #204671
    Purpose
    Mammalian expression of membrane-bound bPAC tagged with paGFP for cAMP production
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Insert
    paGFP-bPAC-CAAX
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    204671-plasmid-map-sequence-id-407793

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  12. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    ICUE4 (RAPGEF3 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181846-plasmid-map-sequence-id-364236

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  13. Epac-S-H74

    Plasmid
    #170338
    Purpose
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:
    Insert
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170338-plasmid-map-sequence-id-335510

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  14. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170349-plasmid-map-sequence-id-344647

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  15. KG#205

    Plasmid
    #110881
    Purpose
    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Asp 448 to Asn
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110881-plasmid-map-sequence-id-212568

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  16. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

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  17. Epac-S-H96

    Plasmid
    #170342
    Purpose
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.
    Insert
    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170342-plasmid-map-sequence-id-344641

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  20. pLenti-CMV-Pink Flamindo-p2a-bPAC

    Plasmid
    #202716
    Purpose
    Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Inserts
    Pink Flamindo
    bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    202716-plasmid-map-sequence-id-402843

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  26. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

    Plasmid
    #202717
    Purpose
    Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Inserts
    Myr-Pink Flamindo
    EGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    202717-plasmid-map-sequence-id-402853

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Showing: 601 - 630 of 3290 results