Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 4161 - 4180 of 11285 results

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pcDNA5/FRT/TO GFP DNAJB2b

Plasmid

#19496

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB2b (DNAJB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-634)

Plasmid

#25718

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-634) (CRTC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA_SZ1-RGG-GFP-RGG

Plasmid

#124932

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Benjamin Schuster, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-HaloTag9

Plasmid

#175546

Purpose

Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphaoB-RLuc8

Plasmid

#140977

Purpose

Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphaoB-Rluc8 (GNAO1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/myc-His-A-YAP1

Plasmid

#19059

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-RAB-EKARev

Plasmid

#118477

Purpose

ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-RAB-EKARev

Use

Tags

N-terminal targeting sequence from Lyn-kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-AT1R-APEX

Plasmid

#96847

Purpose

For expression of AT1R-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

AT1R-APEX (AGTR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-775 USP7

Plasmid

#131247

Purpose

Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_775-1102 USP7

Plasmid

#131250

Purpose

Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-1083 USP7

Plasmid

#131251

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the C-terminal 18 amino acids

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_WT USP7 plasm…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-F98 IRES EGFP

Plasmid

#130710

Purpose

Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP

Use

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 CD mutant

Plasmid

#89896

Purpose

Expresses mouse TET2 CD mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK2

Plasmid

#172616

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK2 (CDK2 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-STIM1-Venus-173-C

Plasmid

#87619

Purpose

Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

STIM1-Venus173-C (STIM1 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA2

Plasmid

#19539

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CMV-Grid2(I677C)

Plasmid

#164782

Purpose

cysteine mutated glutamate delta2 receptor (I677C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Lemoine et al Elife. 2020 Oct 28;9. pii: 59026. doi: 1

Insert

Grid2 I677C (Grid2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

I677C

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-C1483F

Plasmid

#69008

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-C1483F (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

change Cysteine 1483 to Phenylalanine

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only