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Plasmid#119998PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLpC-L-IFP-F[1] (mammalian)
Plasmid#52901PurposeBinary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseRetroviralTagsExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-L-IFP-F[2] (mammalian)
Plasmid#52902PurposeBinary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseRetroviralTagsExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFYF1328 EGFP Site#3
Plasmid#47513Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 3
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-10u
Plasmid#13538DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionBacterialMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_4
Plasmid#78208PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak bindingDepositorInsertmatB (His6 C106A R286C T303A Q457C K488A)
UseTagsHis6ExpressionBacterialMutationC106A R286C T303A Q457C K488APromoterT7Available sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_3
Plasmid#78207PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak bindingDepositorInsertmatB (His6 C106A T167A R286C Q457C K488A)
UseTagsHis6ExpressionBacterialMutationC106A T167A R286C Q457C K488APromoterT7Available sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-10u
Plasmid#13546DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179076PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Scarlet(rfp)-Link-SCS2tmh
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK90 pHomL-TEF2p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)
Plasmid#179074PurposeVector containing a yeast integration cassette for expressing TEF2p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membraneDepositorInsertTEF2p-Scarlet(rfp)-Link-2xPH
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)
Plasmid#179073PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membraneDepositorInsertTDH3p-Scarlet(rfp)-Link-2xPH
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179071PurposeVector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-TOM70-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179069PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisomeDepositorInsertRPL18Bp-PEX25-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179068PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid dropletDepositorInsertRPL18Bp-PDR16-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179065PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuoleDepositorInsertRPL18Bp-PRC1-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)
Plasmid#179063PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosomeDepositorInsertRPL18Bp-Scarlet(rfp)-Link-YPT7
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179062PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartmentDepositorInsertRPL18Bp-SNF7-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR)
Plasmid#179061PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VPS21
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)
Plasmid#179058PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SFT2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)
Plasmid#179057PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SED5
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only