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Plasmid#62326PurposeComplete genome of BoPyV1 isolate S22DepositorInsertFull genome of BoPyV1 isolate S22
UseViral cloneTagsExpressionMutationPromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBluescript SK-rPAI-153
Plasmid#45389DepositorInsertPlasminogen activator inhibitor type 1 (Serpine1 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-rap55
Plasmid#26154DepositorInsertSmed-rap55
UseCloningTagsExpressionMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-eIF3c
Plasmid#26153DepositorInsertSmed-eIF3c
UseCloningTagsExpressionMutationPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
GNE
Plasmid#25255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGNE:T406-R720 (GNE Human)
UseTagsHisExpressionBacterialMutationContains amino acids T406-R720PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZNF289
Plasmid#25232PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertZNF289:A3-A127 (ARFGAP2 Human)
UseTagsHisExpressionBacterialMutationContains amino acids A3-A127PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO SSA1
Plasmid#19462DepositorInsertSSA1 (SSA1 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ1228
Plasmid#11410DepositorInserteukaryotic translation initiation factor
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
AspFlex Kit
Plasmid Kit#1000000217PurposeThis collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.DepositorAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgA2/λ
Plasmid#50371PurposeExpresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
Plasmid#106171Purposecre mediated red to green shiftDepositorInsertsdsRED-express2
eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG
Plasmid#218163PurposeThis plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1
Plasmid#218164PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/κ
Plasmid#52213PurposeHuman immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C
Plasmid#154196PurposeLentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC1-A, sgRNA-BC1-C
UseLentiviral; Catch barcode activationTagsExpressionMammalianMutationPromoterhU6 and mU6Available sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/λ
Plasmid#52214PurposeHuman immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin lambda constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgA1/λ
Plasmid#50370PurposeExpresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG4/λ
Plasmid#50369PurposeExpresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C
Plasmid#154197PurposeLentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC2-A, sgRNA-BC2-C
UseLentiviral; Catch barcode activationTagsExpressionMammalianMutationPromoterhU6 and mU6Available sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG1/λ
Plasmid#50366PurposeExpresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only