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Plasmid#221814PurposeLentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.DepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAC1-V16-hGDNF
Plasmid#122035PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expressionDepositorInserthGDNF (GDNF Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_B.1.1.248
Plasmid#172737PurposeExpression vector for SARS-CoV-2 (B.1.1.248) spike used in the Spike Display systemDepositorInsertSARS-CoV-2 S B.1.1.248 (S Severe acute respiratory syndrome coronavirus 2)
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable sinceAug. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_MAPK6_p.D387N
Plasmid#81712PurposeGateway Donor vector containing MAPK6, part of the Target Accelerator Plasmid Collection.DepositorInsertMAPK6 (MAPK6 Human)
UseGateway entry vectorTagsExpressionMutationL290V; D387N; del amino acids 1-8PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAP2K1_p.E203K
Plasmid#81729PurposeGateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.DepositorInsertMAP2K1 (MAP2K1 Human)
UseGateway entry vectorTagsExpressionMutationL197P; E203K; I310NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HAT1_WT_V5
Plasmid#82933PurposeGateway Donor vector containing HAT1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertHAT1 (HAT1 Human)
UseGateway entry vectorTagsExpressionMutationdeleted amino acids 1 -79, V308APromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1351800.1-COMP-blac-flag-his
Plasmid#111009PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertconserved Plasmodium protein (PF3D7_1351800.1 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1352500-COMP-blac-flag-his
Plasmid#110969PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertthioredoxin-related protein, putative (PF3D7_1352500 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2F04
Plasmid#70901PurposeGateway entry cloneDepositorInsertmod(mdg4) (mod(mdg4) Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mobius Assembly Vector Toolkit
Plasmid Kit#1000000134PurposeMobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate partsDepositorAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo Plant Parts Kit
Plasmid Kit#1000000047PurposeModular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformationDepositorAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
The Mammalian Toolkit
Plasmid Kit#1000000180PurposeA ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.DepositorAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fragmid
Plasmid Kit#1000000237PurposeFragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.DepositorAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
FX cloning Kit
Plasmid Kit#1000000039PurposeInsert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systemsDepositorAvailable sinceApril 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Joung Lab REAL Assembly TALEN Kit
Plasmid Kit#1000000017PurposeUsed to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.DepositorAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
Yeast GPCR-sensor Toolkit
Plasmid Kit#1000000157PurposeThe Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.DepositorAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Yarrowia lipolytica Golden Gate tool kit
Plasmid Kit#1000000167PurposeYarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.DepositorAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Golden Gate TALEN and TAL effector kit 2.0
Plasmid Kit#1000000024PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.DepositorAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Multiplex Yeast Toolkit (MYT)
Plasmid Kit#1000000229PurposeThe Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.DepositorAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only