Addgene

We narrowed to 11,285 results for: pcDNA

Showing: 4141 - 4160 of 11285 results

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pcDNA5/FRT/TO_TOMM20-STf

Plasmid

#182002

Purpose

CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

TOMM20-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2(apf)

Plasmid

#13761

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2(apf) (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif THr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 Y42A/M43A

Plasmid

#19940

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 Y42A/M43A (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a SKP1 binding mutant

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
Yellow Cameleon-Nano65/pcDNA3

Plasmid

#51965

Purpose

mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano65

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-hygro-eGFP-HEC1-9A

Plasmid

#114028

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 9A

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJA2

Plasmid

#19546

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB-Cre-HiBiT

Plasmid

#177912

Purpose

FRB-Cre-HiBiT tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-Cre-HiBiT

Use

Tags

HiBiT tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194378

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA L702P pcDNA3

Plasmid

#206056

Purpose

expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

Depositor

Annette Dolphin

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

L702P

Promoter

CMV

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-R2505P

Plasmid

#69015

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-R2505P (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

change Arginine 2505 to Proline

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Mito-AcGFP-MTn

Plasmid

#154350

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-VSFP-CR

Plasmid

#40257

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

VSFP-CR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP1-S127A

Plasmid

#18988

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein S127A mutant (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Serine 127 changed to Alanine, mutated protein t…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3-deltaBamHI-human MIZ1

Plasmid

#74167

Purpose

Mammalian expression of human MIZ1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MIZ1 (ZBTB17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA-myc-HisA-Sirt3

Plasmid

#24924

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only