-
Plasmid#134449PurposeEmpty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pnos-Cas9-nos
Plasmid#62208PurposeExpresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.DepositorInsertcas9
UseTagsExpressionInsectMutationPromoternanosAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHT-ads
Plasmid#47382PurposeExpresses ads under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
UseTags6xHisExpressionBacterialMutationPromoterPgracAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Reporter-2
Plasmid#180219PurposeSurrogate reporter plasmid for the assay of targeted RNA editingDepositorInsertSurrogate reporter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET302-6His-dCas9-Halo
Plasmid#72269PurposeEncodes a Halo-labeled fusion of nuclease deficient Cas9DepositorInsertHis6-dCas9-Halo
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx162-Hyg
Plasmid#210215PurposeIntegrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert162xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tg (mpx:mCherry-2A-Rac2D57N)
Plasmid#58934Purposeexpresses dominant negative rac2 tagged with mcherry in neutrophilsDepositorInsertmcherry-2a-Rac2D57N (rac2 Zebrafish)
UseTagsmcherry2aExpressionMutationchanged Aspartate 57 to AsparaginePromotermpxAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PIK3R1
Plasmid#174192PurposeExpresses PIK3R1 in mammalian cellsDepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2_8xCTS-ECFP
Plasmid#200249PurposeZebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsert8xCTS-ECFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3R1
Plasmid#174191PurposeExpresses PIK3R1 in mammalian cellsDepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx50-Hyg
Plasmid#210213PurposeIntegrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
LES2A-CRE
Plasmid#167933PurposeLentiviral CRE expression tracked by mScarlet-I fluorescence marker.DepositorInsertmScarlet-P2A-CRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-SP6
Plasmid#102418PurposeAc transposase with SP6 promoter for in vitro transcriptionDepositorInsertAc transposase
UseTagsExpressionBacterialMutationPromoterSP6Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3CG
Plasmid#174189PurposeExpresses PIK3CG in mammalian cellsDepositorInsertPIK3CG (PIK3CG Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Thy1 promoter construct
Plasmid#20736DepositorInsertThy1 promoter (Thy1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T1
Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-cas9-2A-mCherry
Plasmid#107592PurposeAn entry vector with cas9 -2A-mCherryDepositorInsertcas9-2A-mCherry
UseCRISPRTags2A-mCherryExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only