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Plasmid#11529DepositorInsertNAD kinase (AF2373 A. fulgidus)
UseTagsHisTag, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA
Plasmid#74648PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1BA (KDM5B Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationLeu101-(Gly)4-Ala374 deletion mutantPromoterPHAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGC4785
Plasmid#113409PurposeTranslation reporter with inducible translation coupling (low native GFP expression, high under coupling)DepositorInsert359_31121113_2
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationPromoterT7Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sus1/PET32a
Plasmid#26963DepositorInsertSus1 (SUS1 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationwith TEV site to cleave the N-terminal his+Trx tagPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEG608
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
AKAP13
Plasmid#73230PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAKAP13 (AKAP13 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00621:RhoGEF 2002-2193, pfam00169:PH 2236-2336PromoterT7Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
GUCY1B3
Plasmid#39121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGUCY1B3 (GUCY1B1 Human)
UseTagsTEV-His6-FlagExpressionBacterialMutationThis is a complex of two proteins; GUCY1A3 (aa 46…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B3.TM1734
Plasmid#11527DepositorInsertPhoU (TM1734 T. maritima)
UseTagsHisTag, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
UseTagsFlag + eGFPExpressionBacterialMutationPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Atg20-24 pST39
Plasmid#200439PurposeHis6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.DepositorInsertsAtg20
Atg24
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_canine-Caspase-1/4_enzymatic-domain
Plasmid#183383PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0329
Plasmid#162315PurposeTEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiTDepositorInsertT7prom-RBS-NET-TEVprotease-HiBiT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0548
Plasmid#162299PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-TrxA-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0550
Plasmid#162301PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-SUMO-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0539
Plasmid#162306PurposeLevel 0 CDS part for MoClo assembly, AATG-TTCGDepositorInsertCDS, TEV protease (S219V), no stop codon
UseSynthetic BiologyTagsExpressionMutationS219V, codon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0551
Plasmid#162303PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0547
Plasmid#162298PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, TrxA-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0546
Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only