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Plasmid#140979PurposeEncodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaGustducin-RLuc8 (GNAT3 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) rat C/EBP alpha
Plasmid#12550DepositorInsertCCAAT/Enhancer-binding Protein alpha (Cebpa Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4(732)-IgG1
Plasmid#154106PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-PdCO-EGFP
Plasmid#198512PurposeExpresses optimized PdCO in frame with EGFP under control of CMV promotor.DepositorInsertPdCO
UseTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/intron/GFP
Plasmid#113547PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorInsertGFP
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GASTM-3-RVG-10-Lamp2b-HA
Plasmid#71295PurposeEncodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGASTM mutated glycosylation motif, HA, Lamp2 sig…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A CARD8 CARD-FLAG WT
Plasmid#164020PurposeMammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tagDepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO APEX2-eIF4E1
Plasmid#129644PurposeExpress APEX2 fusion to cap-binding protein eIF4E1 in mammalian cellsDepositorInsertEIF4E1 (EIF4E Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-GRK2RH-Nluc
Plasmid#158605PurposePlasmid expressing GRK2(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPA2
Plasmid#19485DepositorInsertheat shock 70kDa protein 2 (HSPA2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 pcDNA1 neo
Plasmid#13580DepositorInsertintegrin beta 6 (ITGB6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (250-536) HA
Plasmid#42209DepositorInsertc-SRC (250-536) HA (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 250-536PromoterCMVAvailable sinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tat101(AD8)-Flag
Plasmid#115777PurposepCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.DepositorInsertHIV-1 Tat protein (AD8)
UseTagsFlagExpressionMammalianMutationNonePromoterCMVAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-SHLD1
Plasmid#114115PurposeExpresses N-terminally tagged FLAG-SHLD1 in mammalian cellsDepositorInsertSHLD1 (SHLD1 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO HIS HSPA1L
Plasmid#19538DepositorInsertheat shock 70kDa protein 1-like (HSPA1L Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc- GFP-Flag
Plasmid#82471PurposeExpresses myc-GFP-FlagDepositorInsertMyc-GFP-Flag
UseTagsFlag and MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
1552 pcDNA3 HA gamma adaptin 1
Plasmid#10712DepositorInsertGamma adaptin 1 (AP1G1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-CAD S1859A
Plasmid#46239PurposeFLAG-HA-CAD with S1859 phosphorylation site mutated to alanineDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
UseTagsFLAG and HAExpressionMammalianMutationSerine 1859 changed to Alanine (S1859A)PromotercmvAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -