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Plasmid#228761PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.DepositorInsertIft88 gRNAs (Ift88 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G13 mutant
Plasmid#231915PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutationDepositorInsertspike G13 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1158QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G14 mutant
Plasmid#231916PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutationDepositorInsertspike G14 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1173QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G15 mutant
Plasmid#231917PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G15 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G145 mutant
Plasmid#231918PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G145 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G1 mutant
Plasmid#231913PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutationDepositorInsertspike G1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G12 mutant
Plasmid#231914PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutationDepositorInsertspike G12 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1134QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4653
Plasmid#215267PurposeTU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoterDepositorInsertpNos:FLP:tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4829
Plasmid#215275PurposeTU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminatorDepositorInsertP35S:NPGA:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHPHS800
Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
UseTagsHygromycin phosphotransferaseExpressionMammalianMutationPromoternoneAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-TJP1
Plasmid#227299PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of TJP1 (TJP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4807
Plasmid#215273PurposeModule for the expressino of the N. nambi HISPS gene under the PNOS promoter and the constitutive expression of EGFP and p19.DepositorInsertPNOS:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4808
Plasmid#215274PurposeModule for the expressino of the N. nambi HISPS gene under the P35S promoter and the constitutive expression of EGFP and p19.DepositorInsertP35S:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4832
Plasmid#215276PurposeModule for the constitutive expression of HISPS, H3H, EGFP and p23.DepositorInsertHISPS-H3H-EGFP-p23
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4485
Plasmid#215258PurposeModule for the constitutive expression of the N. nambi CPH, LUZ, H3H genes, EGFP and p19 fused to a SF in alpha2.DepositorInsertSF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4468
Plasmid#215257PurposeModule for the constitutive expression of the N. nambi LUZ, H3H genes, EGFP and p19 fused to a SF in omega2.DepositorInsertSF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4665
Plasmid#215270PurposeModule for the expression of nptII and the N. nambi LUZ and CPH genes flanked by insulators on the sides of the construction.DepositorInsertMAR10-SF-nptII-LUZ-CPH-MAR10-SF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV107
Plasmid#215337PurposeExpresses sfGFP under induction with the DHBA Marionette Cassette (PcaUAM + P3B5B-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterP3B5BAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-Cas9-2A-GFP
Plasmid#170159PurposeExpress Cas9 and GFP upon doxycycline treatmentDepositorInsertCas9
UseSynthetic Biology; PiggybacTagsGFPExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only