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Plasmid#14648DepositorInsertCdk7 (CDK7 Human)
UseTagsHAExpressionMammalianMutationD155A: dominant negativePromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
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3'-3xFLAG minimal CMV pCDNA5
Plasmid#212045PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha15-RLuc8
Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-nsp7-FLAG
Plasmid#201027PurposeExpresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTags8xHis and FLAGExpressionMammalianMutationPromoterT7 promoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-NLS-TPP1
Plasmid#53585PurposeMammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.DepositorInsertTPP1 (ACD Human)
UseTags3xFLAG and NLSExpressionMammalianMutationSilent mutation to eliminate PacI sitePromoterCMVAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA/Myc DeltaN TCF4
Plasmid#16513DepositorInsertTCF4 (TCF7L2 Human)
UseTagsExpressionMammalianMutationDelta N: lacks aa 1-32PromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
Plasmid#181956PurposeGenetically encoded FRET-based cAMP indicator fused to AKAP79.DepositorInsertAKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
UseTagsAKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eYFP-FKBP-MAD1-WT
Plasmid#114037PurposeChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)DepositorInsertHuman MAD1 (MAD1L1 Human)
UseTagseYFP-FKBP12ExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TDsmURFP-IRES-eGFP
Plasmid#80344PurposeExpresses Tandem Dimer smURFP and eGFP in mammalian cells.DepositorInsertsTandem Dimer smURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKT-PH[R25C]-GFP
Plasmid#18837DepositorInsertAKT-PH domain (AKT1 Human)
UseTagsGFPExpressionMammalianMutationR25CPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet3 CD Mut
Plasmid#72222PurposeExpress flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET3 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT PUR mGSK3A K148A
Plasmid#35158DepositorInsertGSK-3α (Gsk3a Mouse)
UseTagsExpressionMammalianMutationK148A (dominant negative)PromoterCMVAvailable sinceMay 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S (D614G)
Plasmid#215838PurposeExpresses SARS-CoV-2 Spike protein in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR S2035T
Plasmid#26604DepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationS2035TPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorInsertC/EBP beta (Cebpb Mouse)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK6
Plasmid#172614PurposeExpresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cellsDepositorInsertCDK6 (CDK6 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TAT-1-101-FLAG
Plasmid#35981DepositorInserttransactivating protein (tat )
UseTagsFlagExpressionMammalianMutationtat from HIV1 101 amino acidsPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only