-
Plasmid#73946PurposeFor recombinant expression of human LC3A/MAP1LC3A in E. coliDepositorInsertLC3A (MAP1LC3A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAF-WT-tet-puro
Plasmid#199648PurposeDoxycycline-inducible BAF expressionDepositorInsertBAF (BANF1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-REST_sh_seq1_WPRE
Plasmid#127573PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
FOR human OCT4 TALEN
Plasmid#52412PurposeTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)DepositorInsertHD NG NN NN NN HD NG HD NG HD HD HD NI NG
UseTALENTagsExpressionMutationPromoterCAGGSAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-EF1a-FLuc-WPRE-HGHpA
Plasmid#87951PurposeRecombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoterDepositorInsertFirefly Luciferase
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6-sgfiller-eCas9-T2A-BlastR
Plasmid#172437PurposeExpresses eCas9, BlastR and sgRNA cloning siteDepositorInsertseCas9
BlastR
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pMG059 MBP-CREB-His
Plasmid#154076PurposeExpresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
BAF-S4A-tet-puro
Plasmid#199649PurposeDoxycycline-inducible BAF expressionDepositorInsertBAF (BANF1 Human)
UseLentiviralTagsExpressionMutationS4APromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BAF-S4D-tet-puro
Plasmid#199650PurposeDoxycycline-inducible BAF expressionDepositorInsertBAF (BANF1 Human)
UseLentiviralTagsExpressionMutationS4DPromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GATE-16
Plasmid#73518PurposeFor recombinant expression of human GATE-16/GABARAPL2 in E. coliDepositorInsertGATE-16 (GABARAPL2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 1-159
Plasmid#186016Purpose2xHis-LRRK2 Armadillo 1-159DepositorInsert2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
UseTagsExpressionBacterialMutationSee depositor's comments below.PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-LRRK2 Armadillo 350-550
Plasmid#186018Purpose2xHis-LRRK2 Armadillo 350-550DepositorInsert2xHis-LRRK2 Armadillo 350-550 (LRRK2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG024 MBP-GSK3β-HA-His, GST_λPPase
Plasmid#196182PurposeCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCVhyg-HA-FLAG_Ubc9_WT
Plasmid#164936PurposeExpresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNADepositorInserthuman UBC9 (UBE2I Human)
UseRetroviralTagsFLAG & HA tagsExpressionMutationPromoterPGK promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα1(I)
Plasmid#119842PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-HA-Jag1
Plasmid#46052DepositorInsertJag1 (Jag1 Rat)
UseEntry vectorTagsHA tagExpressionMutationPromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only