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Plasmid#208945PurposeA control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-Luc
Plasmid#122034PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expressionDepositorInsertLuciferase
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AKT2 gRNA (BRDN0001148949)
Plasmid#77506Purpose3rd generation lentiviral gRNA plasmid targeting human AKT2DepositorInsertAKT2 (AKT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)PromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAP2K3 gRNA (BRDN0001147291)
Plasmid#76379Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K3DepositorInsertMAP2K3 (MAP2K3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K3 gRNA (BRDN0001146677)
Plasmid#76380Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K3DepositorInsertMAP2K3 (MAP2K3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AKT2 gRNA (BRDN0001147236)
Plasmid#77504Purpose3rd generation lentiviral gRNA plasmid targeting human AKT2DepositorInsertAKT2 (AKT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)
Plasmid#139444PurposeLentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4DepositorInsertFLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationsequence codon-optimizedPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAPK8 gRNA (BRDN0001144736)
Plasmid#78046Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK8DepositorInsertMAPK8 (MAPK8 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK13 gRNA (BRDN0001149329)
Plasmid#76250Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK13DepositorInsertMAPK13 (MAPK13 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK13 gRNA (BRDN0001145247)
Plasmid#76251Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK13DepositorInsertMAPK13 (MAPK13 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK13 gRNA (BRDN0001145708)
Plasmid#76252Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK13DepositorInsertMAPK13 (MAPK13 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK9 gRNA (BRDN0001147698)
Plasmid#76718Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK9DepositorInsertMAPK9 (MAPK9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK11 gRNA (BRDN0001147606)
Plasmid#77482Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK11DepositorInsertMAPK11 (MAPK11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK11 gRNA (BRDN0001146070)
Plasmid#77483Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK11DepositorInsertMAPK11 (MAPK11 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K1 gRNA (BRDN0001146127)
Plasmid#76213Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K1DepositorInsertMAP2K1 (MAP2K1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K3 gRNA (BRDN0001147239)
Plasmid#76381Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K3DepositorInsertMAP2K3 (MAP2K3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK7 gRNA (BRDN0001148313)
Plasmid#76894Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK7DepositorInsertMAPK7 (MAPK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK7 gRNA (BRDN0001145201)
Plasmid#76895Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK7DepositorInsertMAPK7 (MAPK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK7 gRNA (BRDN0001145053)
Plasmid#76896Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK7DepositorInsertMAPK7 (MAPK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only