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pBoBi-hPEN2 D90A
Plasmid#183641PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 90 of PEN2 mutated to alaninePromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 2A
Plasmid#183640PurposeLentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAExpressionMutationresidues 35 and 40 of PEN2 mutated to alaninePromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-RSLID1(WT)
Plasmid#122296Purposeexpresses RSL1D1 protein in mammalian cells (puro resistance)DepositorInsertRSL1D1 (RSL1D1 Human)
UseRetroviralTagsExpressionMammalianMutationPromotervector's LTRAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H
Plasmid#108372PurposeExpresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107578PurposeB. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfbp promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2462
Plasmid#97064PurposeExpressing Sulfolobus solfataricus orf SSO2462 proteinDepositorInsertS. solfataricus orf SSO2462
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2452
Plasmid#97059PurposeExpressing Sulfolobus solfataricus orf SSO2452 proteinDepositorInsertS. solfataricus orf SSO2452
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF126
Plasmid#97021PurposeExpressing Pyrobaculum spherical virus orf126 proteinDepositorInsertPSV orf 126
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEHISSIRV119
Plasmid#97054PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 119 proteinDepositorInsertSIRV orf 119
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEHISSIRV55
Plasmid#97055PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 55 proteinDepositorInsertSIRV orf 55
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA5006
Plasmid#97016PurposeExpressing Pseudomonas aeruginosa orf PA5006 proteinDepositorInsertP aeruginosa orf PA5006
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEANHISECArdB
Plasmid#96967PurposeExpression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tagDepositorInsertE. coli ArdB
UseTagsN-ter TEV cleavable 6HisExpressionBacterialMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA0856
Plasmid#97014PurposeExpressing Pseudomonas aeruginosa orf PA0856 proteinDepositorInsertP aeruginosa orf PA0856
UseTagsN-ter TEV cleavable 6HIsExpressionMutationN-terminal deletion (AA 1-34)PromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4631
Plasmid#97017PurposeExpressing Pseudomonas aeruginosa orf PA4631 proteinDepositorInsertNucleoside-diphosphate-sugar epimerase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSSO2373
Plasmid#97058PurposeExpressing Sulfolobus solfataricus orf SSO2373 proteinDepositorInsertS. solfataricus orf SSO2373
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHISSSO1986
Plasmid#97057PurposeExpressing Sulfolobus solfataricus orf SSO1986 proteinDepositorInsertS. solfataricus orf SSO1986
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPApqsL
Plasmid#97020PurposeExpressing Pseudomonas aeruginosa pqsL proteinDepositorInsertPutative FAD-dependent monooxygenase
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSAPPFK
Plasmid#97003PurposeExpressing MRSA PPFK proteinDepositorInsertPutative phosphofructokinase
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSO1404
Plasmid#97056PurposeExpressing Sulfolobus solfataricus orf SSO1404 proteinDepositorInsertS. solfataricus orf SSO1404
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only
