Addgene

We narrowed to 4,619 results for: Drosophila

Showing: 4061 - 4080 of 4619 results

Results per page:

Sort results by:

gRNA[Sxl].1026B

Plasmid

#112688

Purpose

express gRNA targeting Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Use

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
Dscam1_7.27.25 EC10-Fc-His

Plasmid

#72188

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Dscam1_7.27.25 EC10-AP-His

Plasmid

#72062

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmMextli

Plasmid

#79262

Purpose

Expresses EGFP-tagged DmMextli in S2 cells

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli (mxt Fly, NM_164598)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
R2pH-LAMP-FRB

Plasmid

#225138

Purpose

R2pH-LAMP1-labelled lysosomes with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
RpH-ILV-3xALFA

Plasmid

#225135

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers.

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
R2pH-LAMPx3ALFA

Plasmid

#225136

Purpose

R2pH-LAMP1-labelled lysosomes with 3xLFA

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
RpH-ILV-FRB

Plasmid

#225137

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Plasmid

#217514

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217515

Purpose

Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Plasmid

#217513

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Use

Tags

ALFA tag

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

Plasmid

#201552

Purpose

Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1

Use

Tags

HaloTag

Expression

Insect

Mutation

changed lysine 282 to arginine (numbering based o…

Promoter

Ac5

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p Pcrg1: Khd4-Ada-Gfp (CbxR)

Plasmid

#203734

Purpose

Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, fol

Depositor

Michael Feldbrügge

Article

Sankaranarayanan et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

Khd4-Ada-Gfp

Use

Tags

Gfp

Expression

Bacterial

Mutation

Promoter

Pcrg1

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

Plasmid

#148829

Purpose

Mammalian Expression of HsNot9-V181E-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-V181E-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC

Plasmid

#148475

Purpose

Insect Expression of DmNot1_1149-1376

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1149-1376 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

1 silent mutation compared to the sequence given …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-83-GB1_AD

Plasmid

#148513

Purpose

Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4Ga_617-630-DmThor_64-83 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-DmThor_50-83-Y54AM59A-GB1_AA

Plasmid

#148284

Purpose

Bacterial Expression of DmThor_50-83-Y54AM59A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor_50-83-Y54AM59A (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-DmThor_Z

Plasmid

#148188

Purpose

Bacterial Expression of DmThor

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge