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Plasmid#102639PurposeIn vitro expression of mKalama1 from the T7 promoterDepositorInsertmKalama1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK6 Q203D
Plasmid#80883Purposemammalian expression of ALK6 Q203DDepositorInsertALK6 (Bmpr1b Mouse)
UseTagsHAExpressionMammalianMutationQ203D (constitutively active)PromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM-Asn209Asp_M18
Plasmid#91781PurposeExpression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
UseTagsExpressionMammalianMutationAsn209 changed to Asp (EU numbering)PromoterhEF1-HTLV promAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CyPet
Plasmid#102636PurposeIn vitro expression of CyPet from the T7 promoterDepositorInsertCyPet
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN
Plasmid#85754PurposeExpresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-dCas9
UseCRISPRTagsCsy4 and FokI fusion via 25 amino acid (GGGGS)5 l…ExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX LYCHOS-FLAG-TiD
Plasmid#199747PurposeLentiviral construct to stably express LYCHOS (GPR155) TurboIDDepositorInsertLYCHOS (GPR155 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK6 K231R
Plasmid#80884Purposemammalian expression of ALK6 K231RDepositorInsertALK6 (Bmpr1b Mouse)
UseTagsHAExpressionMammalianMutationK231R (kinase-deficient)PromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN
Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM-Asn209Ala_M18
Plasmid#91733PurposeExpression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
UseTagsExpressionMammalianMutationAsn209 changed to Ala (EU numbering)PromoterhEF1-HTLV promAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-CMV-XRCC1-gRNA res-Neo
Plasmid#176149PurposeXRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassetteDepositorInsertX-ray repair cross complementing 1 (XRCC1 Human)
UseLentiviralTagsExpressionMammalianMutationDual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…PromoterCMVAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
3294_pETcon-SARS2-RBD_Omicron-BA1
Plasmid#184408Purposeyeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationG339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…PromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) FITM2
Plasmid#107504PurposeExpresses FITM2, puromycin resistantDepositorInsertFITM2 (codon optimized)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_hE2F7mg_115mut
Plasmid#73077Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with inserted SNORD115 binding siteDepositorInsertE2F7 (E2F7 Human)
UseTagsExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns, m…PromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRIG-myc-Sema4D-CD4
Plasmid#51606Purposeexpresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backboneDepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseLentiviralTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterRSVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT-cbm
Plasmid#214032PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
UseTagsStrep-tag IIExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-dPCaspase
Plasmid#214035PurposeBacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceumDepositorInsertPCaspase
UseTagsStrep-tag IIExpressionBacterialMutationH79A, C146APromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCaspase-R153A
Plasmid#214036PurposeBacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceumDepositorInsertPCaspase
UseTagsStrep-tag IIExpressionBacterialMutationR153APromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top2A-5X Gly-Venus-T2A-Hygro
Plasmid#211115PurposeFor tagging the C-term of Top2A with VenusDepositorInsertsUseCRISPRTagsExpressionBacterial and MammalianMutationPromotern/aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only