Addgene

We narrowed to 6,564 results for: ski

Showing: 4041 - 4060 of 6564 results

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pAAV-CAG-miRFP2

Plasmid

#178968

Purpose

Expresses miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

AAV

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CAG

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pPN062

Plasmid

#91595

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN063

Plasmid

#91596

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FN-Ets2

Plasmid

#44603

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 (Ets2 Mouse)

Use

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN

Plasmid

#49420

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN (PTEN )

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Fes-SH2

Plasmid

#46426

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fes (FES Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 396-497

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCK341

Plasmid

#192640

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

SoxS has R93A and S101A mutations and dSpRY has s…

Promoter

BBa_J23107 and Sp.pCas9

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Use

Tags

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-eLACCO2.1-NGR

Plasmid

#207989

Purpose

Biosensor for extracellular L-lactate

Depositor

Yuki Kambe, Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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ICAM4-CD4d3+4-bio

Plasmid

#73102

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ICAM4 (ICAM4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp5

Plasmid

#157686

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp5 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp9

Plasmid

#157703

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX SHP-1(C)-SH2

Plasmid

#46494

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(C) (PTPN6 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 105-217

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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ENDOD1-CD4d3+4-bio

Plasmid

#73114

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ENDOD1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ENDOD1 (ENDOD1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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CD55-CD4d3+4-bio

Plasmid

#73116

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD55 (CD55 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Orf6

Plasmid

#157727

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Orf6 (ORF6 SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PuroR-P2A-DD-TCOF1-donor

Plasmid

#60747

Purpose

Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

Depositor

Benhur Lee

Article

Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

Insert

TCOF1 (TCOF1 Human)

Use

Targeting donor

Tags

Expression

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pCIBN-TetR

Plasmid

#103813

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR (CIB1, tetR Mustard Weed, E. coli)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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TRCN0000072225

Plasmid

#78160

Purpose

shLacZ control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

LacZ

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

hU6 and hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only