Addgene

We narrowed to 5,242 results for: pCas

Showing: 4041 - 4060 of 5242 results

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CALNL-RARa-VP16

Plasmid

#185560

Purpose

Cre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Use

Tags

VP16

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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HA-RNF34

Plasmid

#119938

Purpose

RING finger protein 34 expression (HA-tag at the N-terminus)

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only
cPE

Plasmid

#183549

Purpose

Expresses compact Prime Editor in mammalian cells

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

SpCas9 nickase (H840A), Reverse Transcriptase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Use

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Or1a-Gal4

Plasmid

#63019

Purpose

To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

Depositor

Leslie Vosshall

Article

Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

Insert

Drosophila melanogaster Or1a promoter

Use

Tags

Drosophila ADH (aa's 1-18) fused to Yeast Ga…

Expression

Insect

Mutation

Promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only
U6-spTRE3G-CMV-mTagBFP2

Plasmid

#102854

Purpose

A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sp-TRE3G-sgRNA; mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRU51

Plasmid

#167677

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1

Plasmid

#68228

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJK03_Rho_basal_DsRed

Plasmid

#173490

Purpose

Vector backbone used to clone MPRA libraries

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Bovine Rhodopsin promoter driving DsRed fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Bovine Rhodopsin

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pXEE401BG

Plasmid

#91717

Purpose

CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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3924-55

Plasmid

#176499

Purpose

pTRE-EGFP-T2A-TFEB(S3E)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(human; full-length; S3E mutation)-

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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3923-51

Plasmid

#176498

Purpose

pTRE-EGFP-T2A-TFEB(S3A)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(human; full-length; S3A mutation)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-WIPI2d-TEVcs-TSF

Plasmid

#171420

Purpose

For the purifcation of GFP- WIPI2d protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-VAPB(1- 218)- eMagB

Plasmid

#162255

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

TagRFP-T-VAPB(1- 218)- eMagB

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ-177

Plasmid

#195425

Purpose

Transient, constitutive expression of synZiFTR: ZF2-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF2-6 (ZF2) - p65 - RbGpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-179

Plasmid

#195427

Purpose

Transient, constitutive expression of synZiFTR: ZF4-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF4-8 (ZF4) - p65 - RbGpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-182

Plasmid

#195430

Purpose

Transient, constitutive expression of synZiFTR: ZF7-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF7-3 (ZF7) - p65 - RbGpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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