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Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorInsertCD8-NbVHH05-GFP (UBE2J1 Human)
UseTagsCD8 and GFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectMutationPromotermetallothioneinAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-PRSx8-AstR-p2A-GFP
Plasmid#159632PurposeLentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoterDepositorInsertAstR (AstA-R1 Fly)
UseLentiviralTagsGFP and P2AExpressionMammalianMutationPromoterPRSx8Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LN-entry+NLS
Plasmid#128011PurposeVector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag-SV40NLS
UseLamdan entry vector with nls to generate tetherin…TagsLN-3xFlag-SV40NLSExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70
Plasmid#62952PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA-10H-SIII
Plasmid#175447PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsBiP Secretion Peptide and Thrombin-Protein A-10Hi…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70
Plasmid#62953PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9_YTHDF2_sgRNA
Plasmid#186673PurposeYTHDF2 sgRNA plasmidDepositorInsertYTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_Ntag_BbsI-Mammalian
Plasmid#186674PurposeN-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.DepositorInsertMammalian N-terminal Tag
UseTags3xFlag-3xHAExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.hOr47a
Plasmid#126474PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsV5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.mER.hOr47a
Plasmid#126473PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsH. sapiens HCN1 106VNKFSL111 (mER) and V5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
cmv hTid Short H121Q
Plasmid#13710DepositorInserthTid Short H121Q (DNAJA3 Human, hTid Short H121Q)
UseTagsExpressionMammalianMutationH121QPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4C03
Plasmid#70961PurposeGateway entry cloneDepositorInsertHis2Av (His2Av Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry
Plasmid#128014PurposeVector containing the Gal4-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4-DBD-3xFlag
UseGal4 entry vectorTagsGal4-DBD-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
cmv hTid Long H121Q
Plasmid#13708DepositorInserthTid Long H121Q (DNAJA3 Human)
UseTagsExpressionMammalianMutationH121QPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only