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Addgene
Showing: 4021 - 4040 of 25920 results
  1. pAD-GamS

    Plasmid
    #99246
    Purpose
    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
    Depositor
    Insert
    gamS (with C-terminal 6xHis tag)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Availability
    Academic Institutions and Nonprofits only
  2. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. pJK511

    Plasmid
    #71591
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)
    Depositor
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes histidine-222 to glutamine
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pATF1-donor

    Plasmid
    #112328
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF1 with GFP
    Depositor
    Insert
    ATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:50819876:T>A, hg38:12:50819888:T>C,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pFETCh_ATF4

    Plasmid
    #72350
    Purpose
    Donor vector for 3' FLAG tag of human ATF4
    Insert
    ATF4 Homology Arms (ATF4 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pSMPUW_6.10_M3_Ef1a-HA-gateway-SV40

    Plasmid
    #128446
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, and SV40 promoter
    Depositor
    Insert
    Ef1a promoter, N-terminal HA tag, and SV40 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pSMPUW_6.11_M3_Ef1a-gateway-HA-SV40

    Plasmid
    #128447
    Purpose
    lentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoter
    Depositor
    Insert
    Ef1a promoter, C-terminal HA tag, and SV40 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pSMPUW_8.2_M3_CAMKIIA-gateway-V5-SV40

    Plasmid
    #128462
    Purpose
    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, and SV40 promoter
    Depositor
    Insert
    CAMKIIA promoter, C-terminal V5 tag, and SV40 promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. MCS-BioID2-HA pBabe-puro

    Plasmid
    #120308
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    -BioID2-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 4021 - 4040 of 25920 results