Addgene

We narrowed to 12,006 results for: lic

Showing: 4021 - 4040 of 12006 results

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pre-Ubc4 BrG pUC19

Plasmid

#111322

Purpose

for transcription to Ubc4 pre-mRNA

Depositor

Christine Guthrie

Article

Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

Insert

UBC4 (UBC4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

intron has branch-site A to G mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-del(IV)

Plasmid

#111321

Purpose

snu114-del(IV)

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

deletion of domain IVa

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Prp39-pET21b

Plasmid

#111286

Purpose

Prp39 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP39

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETA-Prp28

Plasmid

#111285

Purpose

Prp28 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP28

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Prp40-pET21b

Plasmid

#111287

Purpose

Prp40 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP40

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-Snu114-fSNAPc

Plasmid

#106989

Purpose

open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Snu114

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM1

Plasmid

#106328

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM3

Plasmid

#106327

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM2

Plasmid

#106326

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3MCSpA

Plasmid

#109485

Purpose

Drive inserted gene in osteoblasts.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc PSM4

Plasmid

#106329

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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PoliIFN-γ

Plasmid

#107289

Purpose

Plasmid can be used for secretion of fish Interferon gamma

Depositor

Bohdan Schneider

Article

Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

Insert

Interferon gamma

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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rpaA_NS1

Plasmid

#101829

Purpose

drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with its native promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA

Plasmid

#101830

Purpose

drives expression of rpaA from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::galP_NS1

Plasmid

#101843

Purpose

drives expression of galP transporter from NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Ptrc promoter driving GalP transporter expression

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MetNI (E166Q in MetN)

Plasmid

#78320

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

Inserts

MetI
MetN

Use

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

E166Q

Promoter

T7 Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 to gap

Plasmid

#49158

Purpose

Template for generating gene 60 mRNA, sequence 5' of coding gap only

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

Coding gap and downstream sequence have been del…

Promoter

T7

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylI

Plasmid

#48200

Purpose

Expresses CylI (type III PKS) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylI

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET-29b-CylB

Plasmid

#48199

Purpose

Expresses CylB (acyl carrier protein) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylB

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHYDuet216A

Plasmid

#45611

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE delta variant

Use

Tags

GB1 and H6-GB1

Expression

Bacterial

Mutation

deleted T76, V97, D98, L100 from NpuDnaE intein

Promoter

T7

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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