Addgene

We narrowed to 4,772 results for: codon optimized

Showing: 4021 - 4040 of 4772 results

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pGWKS2

Plasmid

#139410

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

GFP S65T

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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CDS12_Cas9-NLS

Plasmid

#136122

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_AtcoCas9-NLS(noStop)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGWKS17

Plasmid

#139420

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-SpG-His

Plasmid

#179318

Purpose

Plasmid for bacterial expression and purification of SpG-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpG

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_UCP2

Plasmid

#132286

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP2 (UCP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_UCP1

Plasmid

#132274

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP1 (UCP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti‐Cas9‐2A‐Blast

Plasmid

#73310

Purpose

Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-B

Plasmid

#66188

Purpose

expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9pUbi-N

Plasmid

#106331

Purpose

Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

ZmUbi

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_EFS-Cre_ppt-del

Plasmid

#162073

Purpose

Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Insert

Cre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EFS (part of insert)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC16A1

Plasmid

#131934

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A1 (SLC16A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pT3TS-RfxCas13d-HA

Plasmid

#141320

Purpose

Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos, Ariel Bazzini

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

HA

Expression

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-GATA4

Plasmid

#192896

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

GATA4 (GATA4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Codon-optimized

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-G3

Plasmid

#135521

Purpose

Engineered form of fibroblast growth factor 2 with improved thermostability

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

fibroblast growth factor 2 (FGF2 Human)

Use

Tags

6x His tag and Agg Thrombin Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC52A1

Plasmid

#132169

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A1 (SLC52A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC31A1

Plasmid

#132252

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC31A1 (SLC31A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pdCas9-DNMT3A-EGFP

Plasmid

#71666

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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