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Plasmid#162671PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationF495I; codon optimized for expression in E. coli …PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ196
Plasmid#162669PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225ADepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationS225A; codon optimized for expression in E. coli …PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ204
Plasmid#162677PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationC224H, C529F; codon optimized for expression in E…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ201
Plasmid#162674PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationC224W, C529SS; codon optimized for expression in …PromoterT7/lacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK122
Plasmid#74295PurposeProduces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusionDepositorInserthypothetical acetic acid resistance protein B
UseTagsNusAExpressionBacterialMutationPromoterT7Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATCH1
Plasmid#39274DepositorInsertUpdated MCS for pET vectors with enhanced stop codons
UseTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPTDH
Plasmid#66183PurposeExpresses phosphite dehydrogenase in E. coliDepositorInsertPhosphite Dehydrogenase
UseTagsHis6 tagExpressionBacterialMutationE175A, A176RPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoofy12
Plasmid#43977DepositorTypeEmpty backboneUseTagsOneStrepExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy18
Plasmid#43975DepositorTypeEmpty backboneUseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy35
Plasmid#43985DepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
13SA-csy4
Plasmid#97037PurposeSpectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectorsDepositorInsertCys4
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
BAZ2A
Plasmid#73261PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBAZ2A (BAZ2A Human)
UseTagsHis-6-SUMOExpressionBacterialMutationpfam00628:PHD 1652-1696PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KSP_K406
Plasmid#129771PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKSP (kif11.L Frog)
UseTagsHis6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy19
Plasmid#43980DepositorTypeEmpty backboneUseTagsCBPExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC-0_v3
Plasmid#124423PurposeFull MCS (step 2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJ200
Plasmid#162673PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationS136C and a sequence Y75-F104 to introduce a 6th…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ210
Plasmid#162683PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationS136C, G489C, S530C, and a sequence Y75-F104 to i…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ220
Plasmid#162686PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
UseTagsHisExpressionBacterialMutationdeltaG251-T472, C224W, C529S; codon optimized for…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ221
Plasmid#162687PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
UseTagsHisExpressionBacterialMutationdeltaG251-T472, C224H, C529F; codon optimized for…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ217
Plasmid#162685PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
UseTagsHisExpressionBacterialMutationG489C, S530C; codon optimized for E. coli K12PromoterT7/lacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only