Addgene

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TRIN-E-shREN

Plasmid

#105585

Purpose

Dox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTol1:uas-myc-act-map2k2b-2a-tdTomato

Plasmid

#104721

Purpose

UAS-driven expression of activated MEK

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated map2k2b (map2k2b Zebrafish)

Use

Zebrafish transposon

Tags

2A-tdTomato and myc tag

Expression

Mutation

serines 221 and 225 to aspartic acid

Promoter

10xUAS

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pGL421_MLL2_promoter

Plasmid

#118696

Purpose

Express MLL2/KMT2B promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-FL

Plasmid

#105596

Purpose

retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T-Mst1 SARAH

Plasmid

#84297

Purpose

Expresses Mst1 SARAH domain for interaction studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst1 (STK4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
PB-EF1-IRES-NEO mutant ITR

Plasmid

#67905

Purpose

PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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TRIN-E-shTAF12.376

Plasmid

#105573

Purpose

Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
TRIN-shMYB.2572

Plasmid

#105574

Purpose

Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

MYB shRNA #2572

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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TRIN-shREN

Plasmid

#105586

Purpose

Dox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only
PCDNA3-N3FLAG-MYB-(1-327)

Plasmid

#105612

Purpose

transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

N terminal fragment of MYB (mouse), amino acids 1-327 (Myb Mouse)

Use

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-DBD(1-200)

Plasmid

#105610

Purpose

transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

DBD fragment of MYB (mouse), amino acids 1-200 (Myb Mouse)

Use

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3HA-TAF12-HFD(50-130)

Plasmid

#105613

Purpose

transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 HFD amino acid 50-130 with HA tag (Taf12 Mouse)

Use

Tags

3*HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-TAF4(827-909)

Plasmid

#105614

Purpose

transient express TAF4 HFD in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD amino acid 827-909 (Taf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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3H-α-synuclein

Plasmid

#205714

Purpose

Mammalian expression of 3H-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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α-synuclein-3H

Plasmid

#205715

Purpose

Mammalian expression of alpha-synuclein-3H

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

thermostable three helix-bundle protein 3H

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-αS-2

Plasmid

#205706

Purpose

Mammalian expression of alpha-synuclein tandem dimer

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein tandem dimer (SNCA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRSCR

Plasmid

#185345

Purpose

Expression of soluble G2BR with scrambled sequence

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

scrambled gp78 G2BR sequence

Use

Tags

EGFP

Expression

Mammalian

Mutation

scrambled sequence

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSA

Plasmid

#195207

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S162/339/340A

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 3xSD

Plasmid

#195208

Purpose

Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

S162/339/340D

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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