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Plasmid#46781Purposeexpression of WT Rab6a in mammalian cellsDepositorInsertRab6 (RAB6A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP
Plasmid#140877Purpose126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertEGFP
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS
Plasmid#87120PurposeMultisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elementsDepositorInsert4xnrUAS (non repetitive)
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP
Plasmid#75175PurposeTagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertTagBFP
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet-STOP
Plasmid#140876PurposeMultisite Gateway middle entry vector for expression of untagged mScarlet.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-mEos2-NS
Plasmid#115549PurposeMultisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDADepositorInsertLifeact-mEos2
UseTagsExpressionMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab11WT
Plasmid#46785Purposeexpression of WT Rab11a in mammalian cellsDepositorInsertRAB11A (RAB11A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
1456_pDEST_miniTol2_R4-R2_Cryst-eGFP
Plasmid#171795PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorInsertYes associated protein (yap1 Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterSP6Available sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3
Plasmid#75156PurposeNuclear mCerulean2 (blue) fluorophore for use with 5’ promoters.DepositorInsertH2B-mCerulean3
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-mScarlet-NS
Plasmid#140874PurposeMultisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRSM12
Plasmid#101643PurposeEncodes the fluorescent protein meffCFP for constitutive expression in E. coli.DepositorInsertmeffCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
actb2-lifeact-mKate2
Plasmid#109750PurposeUbiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertmKate2 fused to the actin binding lifeact sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5aa
Plasmid#109716PurposeMultisite gateway vector for 3' tagging with rab5aaDepositorInsertrab5aa (rab5aa Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-CaaX(tH)
Plasmid#109539PurposeMultisite gateway vector for 3' tagging with the CaaX domain from HrasDepositorInsertCaaX domain from Hras
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-ccdb-mKate2
Plasmid#109731PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-mKate2-ccdb
Plasmid#109732PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only