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Plasmid#87726PurposeExpresses L195V spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationL195V; 5'UTR deleted (1-190 bp)PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/E112K without 5'UTR
Plasmid#87724PurposeExpresses E112K spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationE112K; 5'UTR deleted (1-190 bp)PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3B/myc-M87E112K
Plasmid#87720PurposeExpresses myc-M87E112K spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM87 isoform (deletion of nt 1-490 - 5'UTR an…PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
nesprin TSmod 25
Plasmid#125555PurposeNesprin-2G force sensor with cerulean-F25-venus TSmoduleDepositorInsertmini-nesprin-2G (Syne2 Mouse)
UseTagscerulean, venusExpressionMammalianMutationTSmod changed from mTFP to cerulean, flageliform …PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-HSPB8
Plasmid#34710DepositorInsertheat shock 22 kDa protein 8 (HSPB8 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 Spastin
Plasmid#134461PurposeM1 Spastin expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationMet to Ala change at amino acid 87PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-SPASTIN
Plasmid#180647PurposeLentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, has siRNA resistance to GAACAGUGUG…PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , residues 108-200
Plasmid#180628PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA, EGFP Spastin M87 with exon 4, N386C
Plasmid#167543PurposeFor doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.DepositorInsertSpastin M87 with exon 4 (SPAST Human)
UseTagsEGFP and HA tagExpressionMammalianMutationN386CPromoterCMVAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , residues 112-196
Plasmid#180627PurposeBacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 112-196PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4
Plasmid#58316PurposeFLAG-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-M11-92
Plasmid#134466PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-Spastin(87-616)DeltaExon4
Plasmid#128794PurposeBacteiral expression of Spastin M87 varient without exon 4DepositorInsertSpastin (SPAST Human)
Use`TagsGSTExpressionBacterialMutationresidues 87-616 without exon 4.PromoterT7Available sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRAF4
Plasmid#58318PurposeEGFP-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 SpastinE442Q
Plasmid#134464PurposeM1 SpastinE442Q expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationGlu to Gln at amino acid 442PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
BACE2_pCSdest
Plasmid#53915DepositorInsertBACE2 (BACE2 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
mApple-M1 SpastinK388R
Plasmid#134463PurposeM1 SpastinK388R expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationLys to Arg at amino acid 388PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK-HA-TRAF4
Plasmid#58314PurposeHA-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Halotag-6xHp
Plasmid#214414PurposeLipid droplet localization of 6xHp of Spastin fused to HalotagDepositorInsert6x Spastin aa 43-92 (SPAST Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-6xHp
Plasmid#214410PurposeLipid droplet localization of 6xHp of Spastin fused to mAppleDepositorInsertSpastin aa 43-92 (SPAST Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only