Addgene

We narrowed to 2,412 results for: organization Addgene

Showing: 381 - 400 of 2412 results

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pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Use

Tags

6xHis and APEX2

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-TSG101

Plasmid

#116925

Purpose

expresses mEGFP-TSG101 in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

TSG101 (TSG101 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-mito

Plasmid

#229692

Purpose

Transient expression of EGFP FP4-mito in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Mitochondira FP4 motifs from Listeria ActA

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-AP4-mito

Plasmid

#229693

Purpose

Transient expression of EGFP AP4-mito in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Control construct with tandem AP4 motifs (mitochondria)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pLX304

Plasmid

#107176

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Lentiviral

Tags

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 mSynCAM 2

Plasmid

#115497

Purpose

expression of mouse full-length SynCAM 2

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm2 (Cadm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pCDNA3

Plasmid

#107174

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pETduet+p300HAT

Plasmid

#157793

Purpose

Expression of p300 HAT domain

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

p300HAT (EP300 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-IG-mSA

Plasmid

#80706

Purpose

Expresses monomeric streptavidin (mSA) in bacterial cells

Depositor

Matthieu Sainlos

Article

Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

Insert

Monomeric streptavidin

Use

Tags

10xHis tag and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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piggybac-EF1-H2AZ-halo

Plasmid

#197907

Purpose

single-particle tracking for H2AZ

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

H2az2 (H2az2 Mouse)

Use

Tags

Halotag

Expression

Mammalian

Mutation

Promoter

EF1

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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ML1Nwt_in_pEGFP-C1

Plasmid

#67797

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo_pAS32

Plasmid

#118222

Purpose

expresses 3xHA-tagged miniTurbo in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

3x HA tag

Expression

Worm

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

ges-1p

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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